LBP Polyclonal antibody

LBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11836-1-AP

Synonyms

Lipopolysaccharide-binding protein

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Validation Data Gallery

• 

  WB analysis of mouse liver using 11836-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11836-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11836-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11836-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11836-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 11836-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11836-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11836-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11836-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 11836-1-AP

  IP result of anti-LBP (IP:11836-1-AP, 4ug; Detection:11836-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human liver using 11836-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 11836-1-AP (LBP Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 11836-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 11836-1-AP (LBP Antibody) at dilution of 1:50.

• 

  IF Staining of L02 using 11836-1-AP

  Immunofluorescent analysis of L02 cells using 11836-1-AP (LBP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11836-1-AP

  Immunofluorescent analysis of HepG2 cells, using LBP antibody 11836-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse liver tissue, A431 cells, A549 cells, HeLa cells, Jurkat cells, L02 cells, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: L02 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

11836-1-AP targets LBP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LBP fusion protein Ag2431
• Full Name: lipopolysaccharide binding protein
• Calculated Molecular Weight: 481 aa, 53 kDa
• Observed Molecular Weight: 63-67 kDa
• GenBank Accession Number: BC022256
• Gene Symbol: LBP
• Gene ID (NCBI): 3929
• RRID: AB_2097479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LBP belongs to the BPI/LBP/Plunc superfamily and BPI/LBP family. It binds to the lipid A moiety of bacterial lipopolysaccharides (LPS) which is a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In many tissue/cells, we get 53-60 kDa and 63-67 kDa(Glycosylation form).

Publications

Species	Application	Title
		J Neurosurg; Association of decreased levels of lipopolysaccharide-binding protein with OKN-007-induced regression of tumor growth in an F98 rat glioma model.; Authors - Nataliya Smith
human	WB	Cancer Sci; Circulating exosomes contain protein biomarkers of metastatic non-small-cell lung cancer.; Authors - Ning Wang
rat	IHC	J Biomed Mater Res A; The characteristic regulation of gene expression Lbp and Sod3 in peri-implant connective tissue of rats.; Authors - Takafumi Kobayashi