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  • Western Blot (WB) analysis of various lysates using LASS2 Recombinant antibody (84514-4-RR)

    WB analysis using 84514-4-RR (same clone as 84514-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84514-4-RR (LASS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84514-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84514-4-RR

    Affinity and Kinetic Characterization of 84514-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84514-4-RR against Human LASS2 were performed. The affinity constant is 0.549 nM.

LASS2 Recombinant antibody, PBS Only

LASS2 {ptg:Brand} Recombinant Antibody for WB, Indirect ELISA
Cat No. 84514-4-PBS
Clone No.241898E11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Sphingosine N-acyltransferase CERS2, EC:2.3.1.297, EC:2.3.1.24, CerS2, Ceramide synthase 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Product Information

84514-4-PBS targets LASS2 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LASS2 fusion protein Ag14151 Predict reactive species
Full Name LAG1 homolog, ceramide synthase 2
Calculated Molecular Weight 380 aa, 45 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC010032
Gene Symbol LASS2
Gene ID (NCBI) 29956
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96G23
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

LASS2, also known as TMSG1, is a novel suppressor of human cancer metastasis. As one member of LASS family, including LASS1-6, LASS2 mRNA is at the highest level of all LASS members, and has the broadest tissue distribution, particularly abundant in the liver, kidney and brain in mice. The biological roles of LASS2 include protection from aging , hepatic INS resistance, and hepatocellular carcinoma (HCC) progression. LASS2 has been correlated with the degree of invasion and recurrence of carcinomas of the prostate, liver, breast and bladder.