LASP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

LASP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

29

Reviews (1)

Cat no : 10515-1-AP

Synonyms

MLN50, LIM and SH3 protein 1, LASP-1

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Validation Data Gallery

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  WB analysis of PC-3 using 10515-1-AP

  WB result of LASP1 antibody (10515-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LASP1 transfected PC-3 cells.

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  WB analysis using 10515-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10515-1-AP (LASP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 10515-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10515-1-AP (LASP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10515-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10515-1-AP (LASP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10515-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10515-1-AP (LASP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 10515-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10515-1-AP (LASP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 10515-1-AP

  IP result of anti-LASP1 (IP:10515-1-AP, 4ug; Detection:10515-1-AP 1:8000) with A549 cells lysate 600 ug.

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  IHC staining of human breast cancer using 10515-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10515-1-AP (LASP1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 10515-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10515-1-AP (LASP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10515-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10515-1-AP (LASP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10515-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10515-1-AP (LASP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10515-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10515-1-AP (LASP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10515-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10515-1-AP (LASP1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of A549 using 10515-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using LASP1 antibody (10515-1-AP) at dilution of 1:1600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, MDA-MB-453s cells, mouse brain tissue, PC-3 cells, MCF-7 cells, Jurkat cells, SGC-7901 cells, SKOV-3 cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 26 publications below
• IHC: See 12 publications below
• IF: See 7 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Product Information

10515-1-AP targets LASP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LASP1 fusion protein Ag0800
• Full Name: LIM and SH3 protein 1
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC012460
• Gene Symbol: LASP1
• Gene ID (NCBI): 3927
• RRID: AB_2280966
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LASP1(LIM and SH3 protein 1), also known as MLN50, is a 261 amino acid protein that localizes to both the cytoplasm and the cytoskeleton(PMID: 7589475). LASP1 consists of an N-terminal LIM-domain with two zinc finger motifs, followed by two central actin-binding nebulin repeats, flanked by a linker region and a C-terminal SH3 domain (PMID: 17177073, 9848085). LASP-1 interacts with F-Actin and plays an important role in the regulation of Actin-associated cytoskeletal organization. Agonist-dependent changes in LASP1 phosphorylation may regulate Actin-related ion transport activities in epithelial cells (PMID: 15465019,12571245). Overexpression of LASP-1 is associated with breast cancer, and plays a role in tumor transformation and metastasis (PMID: 17956604).

Publications

Species	Application	Title
	IP	Cell Host Microbe; Global mapping of Salmonella enterica-host protein-protein interactions during infection.; Authors - Philipp Walch
human	WB	J Exp Clin Cancer Res; RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization; Authors - Shuang Tao
human	WB,IHC	Oncogene; LASP1 interacts with N-WASP to activate the Arp2/3 complex and facilitate colorectal cancer metastasis by increasing tumour budding and worsening the pattern of invasion.; Authors - Pingping Yan
human	WB	Oncotarget; Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma.; Authors - Hao Bo
human	WB,IHC,IF,CoIP	Oncogenesis; CCT8 recovers WTp53-suppressed cell cycle evolution and EMT to promote colorectal cancer progression.; Authors - Qing Liao ; KD Validated
human	WB,IHC,IF	Cancer Lett; LASP-1 induces proliferation, metastasis and cell cycle arrest at the G2/M phase in gallbladder cancer by down-regulating S100P via the PI3K/AKT pathway.; Authors - ZhiZhen Li ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (06-03-2019)

Lanes show molecular weight, then cells expressing GFP-LASP1, then wildtype cells. This antibody works well, staining both endogenous and exogenous LASP1.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

LASP1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10515-1-AP)