LAS1L Polyclonal antibody

LAS1L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16010-1-AP

Synonyms

Ribosomal biogenesis protein LAS1L, Protein LAS1 homolog, MSTP060, Endoribonuclease LAS1L, EC:3.1.-.-

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Validation Data Gallery

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  WB analysis using 16010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16010-1-AP (LAS1L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16010-1-AP

  IP result of anti-LAS1L (IP:16010-1-AP, 4ug; Detection:16010-1-AP 1:1000) with HEK-293 cells lysate 1200ug.

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  IHC staining of human ovary cancer using 16010-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16010-1-AP (LAS1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16010-1-AP

  Immunofluorescent analysis of HepG2 cells using 16010-1-AP (LAS1L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HEK-293 cells, HeLa cells, HepG2 cells, PC-3 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

16010-1-AP targets LAS1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LAS1L fusion protein Ag8732
• Full Name: LAS1-like (S. cerevisiae)
• Calculated Molecular Weight: 734 aa, 83 kDa
• Observed Molecular Weight: 83 kDa, 70-75 kDa
• GenBank Accession Number: BC014545
• Gene Symbol: LAS1L
• Gene ID (NCBI): 81887
• RRID: AB_2132810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAS1L is a nucleolar protein required for the synthesis of the 60S ribosomal subunit and maturation of the 28S rRNA. LAS1L interacts with PELP1, TEX10, and WDR18, along with NOL9 and SENP3, to form a novel nucleolar complex that cofractionates with the 60S preribosomal subunit.

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation.; Authors - Fanis Pavlos P
human	WB	Cancer Res Commun; PELP1 is a novel therapeutic target in hepatocellular carcinoma; Authors - Khaled Mohamed Nassar
human	WB	Breast Cancer Res Treat; Targeting PELP1 oncogenic signaling in TNBC with the small molecule inhibitor SMIP34; Authors - Kristin A Altwegg
human	WB	Mol Oncol; PELP1 inhibition by SMIP34 reduces endometrial cancer progression via attenuation of ribosomal biogenesis; Authors - Xue Yang