LARS Polyclonal antibody

• Featured Product
• KD/KO Validated

LARS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 21146-1-AP

Synonyms

hr025Cl, HSPC192, KIAA1352, LARS, LARS1, Leucine tRNA ligase, leucyl tRNA synthetase, LEURS, LEUS, LRS, PIG44, RNTLS

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Validation Data Gallery

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  WB analysis using 21146-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21146-1-AP (LARS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21146-1-AP

  WB result of LARS antibody (21146-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LARS transfected HeLa cells.

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  IP experiment of Jurkat using 21146-1-AP

  IP result of anti-LARS (IP:21146-1-AP, 4ug; Detection:21146-1-AP 1:500) with Jurkat cells lysate 2400ug.

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  IHC staining of human colon using 21146-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21146-1-AP (LARS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 21146-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21146-1-AP (LARS Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 21146-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LARS antibody (21146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 2 publications below

Product Information

21146-1-AP targets LARS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LARS fusion protein Ag15684
• Full Name: leucyl-tRNA synthetase
• Calculated Molecular Weight: 1176 aa, 134 kDa
• Observed Molecular Weight: 135-140 kDa
• GenBank Accession Number: BC151214
• Gene Symbol: LARS
• Gene ID (NCBI): 51520
• RRID: AB_10733878
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Autophagy; KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.; Authors - Guokai Yan
human	WB	PLoS One; A Genome-Wide siRNA Screen in Mammalian Cells for Regulators of S6 Phosphorylation.; Authors - Angela Papageorgiou
human	IHC	Biomed Res Int; Dickkopf 4 Alone and in Combination with Leucyl-tRNA Synthetase as a Good Prognostic Biomarker for Human Colorectal Cancer; Authors - Su-Jeong Park
human	WB	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj
human	WB,IHC	J Transl Med; Identification of LARS as an essential gene for osteosarcoma proliferation through large-Scale CRISPR-Cas9 screening database and experimental verification; Authors - Wenhao Chen ; KD Validated
human	WB	Proc Natl Acad Sci U S A; Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex; Authors - Krishnendu Khan ; KD Validated