LARP2 Polyclonal antibody

LARP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17277-1-AP

Synonyms

La related protein 1B, La related protein 2, LARP1B, LARP2

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Validation Data Gallery

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  WB analysis using 17277-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17277-1-AP (LARP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 17277-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17277-1-AP (LARP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 17277-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17277-1-AP (LARP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, PC-3 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

The immunogen of 17277-1-AP is LARP2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LARP2 fusion protein Ag11043
• Full Name: La ribonucleoprotein domain family, member 2
• Calculated Molecular Weight: 201 aa, 24 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC030516
• Gene Symbol: LARP2
• Gene ID (NCBI): 55132
• RRID: AB_2918052
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The La-related proteins (LARPs) share a conserved RNA-binding unit composed of a La Motif and an RNA Recognition Motif (RRM), which recognizes diverse RNA substrates. LARP2 has nine isoforms with MW 30-40 kDa, 90-105 kDa. Alternative splicing has been observed at this locus and multiple variants, encoding distinct isoforms, are described. Additional splice variation has been identified but the full-length nature of these transcripts has not been determined. (PMID: 26501340, PMID: 33292040)

Publications

Species	Application	Title
human	IHC	Transl Oncol; Induction of LARP1B under endoplasmic reticulum stress and its regulatory role in proliferation of esophageal squamous cell carcinoma; Authors - Xia Yang