LARP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

LARP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, drosophila

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13708-1-AP

Synonyms

LARP, La-related protein 1, La related protein 1, KIAA0731

---

Validation Data Gallery

• 

  WB analysis of HEK-293T using 13708-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13708-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13708-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 13708-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13708-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13708-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 13708-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 13708-1-AP (LARP1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 13708-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 13708-1-AP (LARP1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 13708-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 13708-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13708-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13708-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LARP1 antibody (13708-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, HepG2 cells, mouse heart tissue, mouse testis tissue, mouse brain tissue
• Positive IHC detected in: human ovary cancer tissue, human liver cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:800-1:3200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

13708-1-AP targets LARP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, drosophila samples.

• Tested Reactivity: human, mouse, rat, drosophila
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LARP1 fusion protein Ag4645
• Full Name: La ribonucleoprotein domain family, member 1
• Calculated Molecular Weight: 1096 aa, 124 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC033856
• Gene Symbol: LARP1
• Gene ID (NCBI): 23367
• RRID: AB_2296670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Sci Adv; The 40S-LARP1 complex reprograms the cellular translatome upon mTOR inhibition to preserve the protein synthetic capacity.; Authors - Pedro Fuentes ; KO Validated
human	WB	Mol Cell Proteomics; A new cellular interactome of SARS-CoV-2 nucleocapsid protein and its biological implications; Authors - Yuan-Qin Min
mouse	WB	Mol Autism; Ribosome profiling in mouse hippocampus: plasticity-induced regulation and bidirectional control by TSC2 and FMRP.; Authors - Annie Hien