LAMR1/RPSA Recombinant antibody, PBS Only

LAMR1/RPSA Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240461G11

Cat no : 83495-7-PBS

Synonyms

LAMR1,RPSA, RPSA, 67LR, 67 kDa laminin receptor, 40S ribosomal protein SA

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Validation Data Gallery

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  WB analysis using 83495-7-RR (same clone as 83495-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83495-7-RR (LAMR1/RPSA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83495-7-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83495-7-RR (same clone as 83495-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LAMR1/RPSA antibody (83495-7-RR, Clone: 240461G11 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83495-7-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 83495-7-RR (same clone as 83495-7-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug LAMR1/RPSA Recombinant antibody (83495-7-RR, Clone:240461G11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83495-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83495-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83495-7-RR against Human LAMR1,RPSA were performed. The affinity constant is 1.14 nM.

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Product Information

The immunogen of 83495-7-PBS is LAMR1/RPSA Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LAMR1/RPSA fusion protein Ag6033
• Full Name: ribosomal protein SA
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC050688
• Gene Symbol: RPSA
• Gene ID (NCBI): 3921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The ribosomal protein SA (RPSA), previously named 67 kD laminin receptor(67LR), 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein, is a multifunctional protein, that plays a role in a number of pathological processes, such as cancer and prion diseases. It is overexpressed in various cancer cell lines, and the the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. This antibody is a rabbit polyclonal antibody raised against full length RPSA of human origin. This antibody is specific to RPSA (LAMR1).67LR derives from homo- or hetero- dimerization of a 37 kDa cytosolic precursor (37LRP)], most probably by fatty acid acylation. 37LRP is mostly found in the cytosol and nucleus [10] where it is involved in translational processes and maintenance of nuclear structures, respectively. 67LR is localized at the cell surface and it not only serves as a receptor for LM but also acts as a receptor for elastin, carbohydrates and the cellular prion protein.