LAMR1/RPSA Polyclonal antibody

• Featured Product
• KD/KO Validated

LAMR1/RPSA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 14533-1-AP

Synonyms

LAMR1,RPSA, RPSA, Colon carcinoma laminin-binding protein, 67LR, 67 kDa laminin receptor

---

Validation Data Gallery

• 

  WB analysis using 14533-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14533-1-AP (LAMR1/RPSA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14533-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14533-1-AP (LAMR1,RPSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 14533-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14533-1-AP (LAMR1,RPSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 14533-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14533-1-AP (LAMR1/RPSA antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14533-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LAMR1/RPSA antibody (14533-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 14533-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human LAMR1/RPSA (14533-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: COLO 320 cells, mouse liver tissue, mouse colon tissue, HeLa cells, NIH/3T3 cells, PC-12 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

14533-1-AP targets LAMR1/RPSA in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LAMR1/RPSA fusion protein Ag6033
• Full Name: ribosomal protein SA
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC050688
• Gene Symbol: RPSA
• Gene ID (NCBI): 3921
• RRID: AB_2182528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ribosomal protein SA (RPSA), previously named 67 kD laminin receptor(67LR), 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein, is a multifunctional protein, that plays a role in a number of pathological processes, such as cancer and prion diseases. It is overexpressed in various cancer cell lines, and the the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. This antibody is a rabbit polyclonal antibody raised against full length RPSA of human origin. This antibody is specific to RPSA (LAMR1).67LR derives from homo- or hetero- dimerization of a 37 kDa cytosolic precursor (37LRP)], most probably by fatty acid acylation. 37LRP is mostly found in the cytosol and nucleus [10] where it is involved in translational processes and maintenance of nuclear structures, respectively. 67LR is localized at the cell surface and it not only serves as a receptor for LM but also acts as a receptor for elastin, carbohydrates and the cellular prion protein.

Publications

Species	Application	Title
	IF	Neuron; Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome.; Authors - Roberta Cagnetta
mouse	WB	Arterioscler Thromb Vasc Biol; β1-Integrin Is Essential for Vasoregulation and Smooth Muscle Survival In Vivo.; Authors - Turlo Kirsten A KA
human	WB	Virulence; LAMR1 restricts Zika virus infection by attenuating the envelope protein ubiquitination.; Authors - Dingwen Hu ; KD Validated
mouse	WB	Hum Gene Ther; Strategies to Attenuate Myocardial Infarction and No-Reflow through Preservation of Vascular Integrity by Pigment Epithelium-derived Factor.; Authors - Hao Zhang
mouse	WB	Sci Rep; Epigallocatechin-3-gallate inhibits H2O2-induced apoptosis in Mouse Vascular Smooth Muscle Cells via 67kD Laminin Receptor.; Authors - Xue Yan
human,mouse	WB,IHC	Food Funct; Theaflavin-3,3'-di-gallate represses prostate cancer by activating the PKCδ/aSMase signaling pathway through a 67 kDa laminin receptor.; Authors - Lingli Sun