LAMB2 Polyclonal antibody

LAMB2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30943-1-AP

Synonyms

S LAM beta, LAMS, Laminin 3 subunit beta, Laminin 15 subunit beta, Laminin 14 subunit beta

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Validation Data Gallery

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  WB analysis using 30943-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30943-1-AP (LAMB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30943-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30943-1-AP (LAMB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30943-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 30943-1-AP (LAMB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 30943-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30943-1-AP (LAMB2 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse heart tissue, mouse kidney tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30943-1-AP targets LAMB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LAMB2 fusion protein Ag33468
• Full Name: laminin, beta 2 (laminin S)
• Calculated Molecular Weight: 196 kDa
• Observed Molecular Weight: 195-200 kDa
• GenBank Accession Number: NM_002292.3
• Gene Symbol: LAMB2
• Gene ID (NCBI): 3913
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAMB2 (laminin subunit beta 2), also known as LAMS. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. This gene encodes the beta chain isoform laminin, beta 2. It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle. Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination, showed defects in the maturation of neuromuscular junctions and impairment of glomerular filtration. The calculated molecular weight of LAMB2 is 200 kDa.