LAMA1 Polyclonal antibody

LAMA1 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28697-1-AP

Synonyms

Laminin 1 subunit alpha, laminin, alpha 1, LAMA, Laminin subunit alpha-1, Laminin subunit alpha 1

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Validation Data Gallery

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  WB analysis using 28697-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28697-1-AP (LAMA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of rat colon using 28697-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using LAMA1 antibody (28697-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, U2OS cells
• Positive IF-P detected in: rat colon tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28697-1-AP targets LAMA1 in WB, IF-P, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LAMA1 fusion protein Ag29329
• Full Name: laminin, alpha 1
• Calculated Molecular Weight: 337 kDa
• Observed Molecular Weight: 337 kDa
• GenBank Accession Number: NM_005559
• Gene Symbol: LAMA1
• Gene ID (NCBI): 284217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAMA1 is also known as laminin subunit α1. Laminins are a family of glycoproteins found in the extracellular matrix that comprises the basement membrane. Laminins have a heterotrimeric structure composed of an α, β, and γ chain. Numerous biological processes are known to be directed by them, including cell adhesion, mitogenesis, differentiation, and metastasis, all of which contribute to carcinogenesis. Tissue distribution of LAMA1 occurred mainly in early epithelial development and some adult epithelia. Recent reports have shown that laminin-1 acts as an efficient attachment protein for a large variety of cultured cell types in vitro. (PMID: 36824663)