LAIR1 Polyclonal antibody

LAIR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13297-1-AP

Synonyms

Leukocyte-associated immunoglobulin-like receptor 1, LAIR-1, LAIR 1, hLAIR1, CD305

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Validation Data Gallery

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  WB analysis using 13297-1-AP

  HeLa cells (LAIR1 negative) and THP-1 cells were subjected to SDS PAGE followed by western blot with 13297-1-AP (LAIR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 13297-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13297-1-AP (LAIR1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: THP-1 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13297-1-AP targets LAIR1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LAIR1 fusion protein Ag4021
• Full Name: leukocyte-associated immunoglobulin-like receptor 1
• Calculated Molecular Weight: 287 aa, 31 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC027899
• Gene Symbol: LAIR1
• Gene ID (NCBI): 3903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) is a transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs (PMID: 9285412). LAIR1 is expressed on the majority of human PBMCs, including NK, T, B, monocytes, and dendritic cells, as well as the majority of thymocytes (PMID: 10229813). It functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of NK cells, B cells and T cells. LAIR1 is a collagen receptor (PMID: 16754721). Tumor-expressed collagens can modulate immune cell function through the inhibitory collagen receptor LAIR1 (PMID: 21955987).