LAG-3/CD223 Polyclonal antibody

LAG-3/CD223 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 29548-1-AP

Synonyms

LAG-3, CD223, lymphocyte activation gene 3, LAG3, LAG 3

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Validation Data Gallery

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  IHC staining of human tonsillitis using 29548-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29548-1-AP (LAG-3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 29548-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29548-1-AP (LAG-3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

29548-1-AP targets LAG-3/CD223 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: fusion protein
• Full Name: lymphocyte-activation gene 3
• Calculated Molecular Weight: 57 kDa
• GenBank Accession Number: NM_002286
• Gene Symbol: LAG-3
• Gene ID (NCBI): 3902
• RRID: AB_2918324
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)

Publications

Species	Application	Title
human	IHC	Front Immunol; Prognostic value and immune landscapes of TERT promoter methylation in triple negative breast cancer; Authors - Fei Lin
human	IHC	Eur J Med Res; A combined analysis of multi-omics data reveals the prognostic values and immunotherapy response of LAG3 in human cancers; Authors - Jinwu Peng