L2HGDH Monoclonal antibody, PBS Only

L2HGDH Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D11C2

Cat no : 66269-1-PBS

Synonyms

C14orf160, DURANIN, L2HGDH

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Validation Data Gallery

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  WB analysis of pig brain using 66269-1-Ig (same clone as 66269-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66269-1-Ig (L2HGDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66269-1-Ig (same clone as 66269-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66269-1-Ig (L2HGDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66269-1-Ig (same clone as 66269-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66269-1-Ig (L2HGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66269-1-Ig (same clone as 66269-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66269-1-Ig (L2HGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66269-1-Ig (same clone as 66269-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using L2HGDH antibody (66269-1-Ig, Clone: 3D11C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66269-1-Ig (same clone as 66269-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using L2HGDH antibody (66269-1-Ig, Clone: 3D11C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

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Product Information

66269-1-PBS targets L2HGDH in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: L2HGDH fusion protein Ag8382
• Full Name: L-2-hydroxyglutarate dehydrogenase
• Calculated Molecular Weight: 463aa,50 kDa; 441aa,49 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC006117
• Gene Symbol: L2HGDH
• Gene ID (NCBI): 79944
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

L2HGDH(L-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as duranin, C14orf160 and belongs to the L2HGDH family. The putative L2HGDH is predicted to be targeted to the mitochondria where its mitochondrial targeting sequence is presumably removed(PMID:16005139). Defects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA). It has 2 isoforms produced by alternative splicing with the molecular weight of 50 kDa and 48 kDa. L2HGDH also can be detected as ~45kD due to the 51aa transit peptide cleaved.