L2HGDH Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

L2HGDH Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15707-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 15707-1-AP (same clone as 15707-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15707-1-AP (same clone as 15707-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse small intestine using 15707-1-AP (same clone as 15707-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

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  WB analysis of SGC-7901 using 15707-1-AP (same clone as 15707-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 15707-1-AP (same clone as 15707-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH Antibody) at dilution of 1:800 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15707-1-AP (same clone as 15707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15707-1-AP (same clone as 15707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 15707-1-AP (same clone as 15707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 15707-1-AP (same clone as 15707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

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Product Information

15707-1-PBS targets L2HGDH in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: L2HGDH fusion protein Ag8382
• Full Name: L-2-hydroxyglutarate dehydrogenase
• Calculated Molecular Weight: 463aa,50 kDa; 441aa,49 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC006117
• Gene Symbol: L2HGDH
• Gene ID (NCBI): 79944
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

L2HGDH(L-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as duranin, C14orf160 and belongs to the L2HGDH family. The putative L2HGDH is predicted to be targeted to the mitochondria where its mitochondrial targeting sequence is presumably removed(PMID:16005139). Defects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA). It has 2 isoforms produced by alternative splicing with the molecular weight of 50 kDa and 48 kDa. L2HGDH also can be detected as ~45kD due to the 51aa transit peptide cleaved.