L1TD1 Polyclonal antibody

L1TD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21528-1-AP

Synonyms

LINE-1 type transposase domain-containing protein 1, ES cell-associated protein 11, ES cell associated protein 11, ECAT11

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Validation Data Gallery

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  WB analysis of NCCIT cells using 21528-1-AP

  WB result of L1TD1 antibody (21528-1-AP, 1:500) with NCCIT cells.

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  IHC staining of human placenta using 21528-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21528-1-AP (L1TD1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 21528-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21528-1-AP (L1TD1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary cancer using 21528-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 21528-1-AP (L1TD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 21528-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 21528-1-AP (L1TD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 21528-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21528-1-AP (L1TD1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 21528-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21528-1-AP (L1TD1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of NCCIT using 21528-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NCCIT cells using L1TD1 antibody (21528-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: NCCIT cells
• Positive IHC detected in: human ovary cancer tissue, human ovary tumor tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NCCIT cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21528-1-AP targets L1TD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: L1TD1 fusion protein Ag16147
• Full Name: LINE-1 type transposase domain containing 1
• Calculated Molecular Weight: 865 aa, 99 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC111559
• Gene Symbol: L1TD1
• Gene ID (NCBI): 54596
• RRID: AB_2878878
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

L1TD1 is an RNA-binding protein highly and specifically expressed in pluripotent cells. It is essential for maintaining the pluripotentency in human embryonic stem cells (hESCs) and has been considered as a marker for undifferentiated hESCs. L1TD1 is also reported to be highly expressed in medulloblastoma cells and its expression in medulloblastoma correlates with poor clinical outcome.