L1CAM Recombinant antibody

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  IF Staining of SH-SY5Y using 84034-5-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using L1CAM antibody (84034-5-RR, Clone: 240978A7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84034-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84034-5-RR against Human L1CAM were performed. The affinity constant is 0.522 nM.

L1CAM Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Cat No. 84034-5-RR

Clone No.240978A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

L1 cell adhesion molecule, HSAS1, HSAS, CD171, CAML1

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Tested Applications

• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84034-5-RR targets L1CAM in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: L1 cell adhesion molecule
• Calculated Molecular Weight: 1257 aa, 140 kDa
• GenBank Accession Number: BC126229
• Gene Symbol: L1CAM
• Gene ID (NCBI): 3897
• RRID: AB_3671603
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P32004
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).