L1CAM Monoclonal antibody, PBS Only

L1CAM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat, Rabbit

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H7B9

Cat no : 67115-1-PBS

Synonyms

CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule, L1CAM, MASA, MIC5, N CAM L1, N CAML1, NCAM L1, S10, SPG1

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Validation Data Gallery

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  WB analysis using 67115-1-Ig (same clone as 67115-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67115-1-Ig (L1CAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

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  WB analysis of pig cerebellum using 67115-1-Ig (same clone as 67115-1-PBS)

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67115-1-Ig (L1CAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 67115-1-Ig (same clone as 67115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 67115-1-Ig (same clone as 67115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L1CAM antibody (67115-1-Ig, Clone: 3H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L1CAM antibody (67115-1-Ig, Clone: 3H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

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Product Information

67115-1-PBS targets L1CAM in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Pig, Mouse, Rat, Rabbit samples.

• Tested Reactivity: Human, Pig, Mouse, Rat, Rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: L1CAM fusion protein Ag17706
• Full Name: L1 cell adhesion molecule
• Calculated Molecular Weight: 1257 aa, 140 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC126229
• Gene Symbol: L1CAM
• Gene ID (NCBI): 3897
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).