L1CAM Polyclonal antibody

L1CAM Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 20659-1-AP

Synonyms

CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule, L1CAM, MASA, MIC5, N CAM L1, N CAML1, NCAM L1, S10, SPG1

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Validation Data Gallery

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  WB analysis using 20659-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20659-1-AP (L1CAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

20659-1-AP targets L1CAM in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: L1 cell adhesion molecule
• Calculated Molecular Weight: 140 kDa
• Observed Molecular Weight: 200-220 kDa, 80 kDa
• GenBank Accession Number: NM_000425
• Gene Symbol: L1CAM
• Gene ID (NCBI): 3897
• RRID: AB_2878717
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503). It has been reported that L1CAM can be proteolytically cleaved into a soluble fragment of 140 kDa and an intracellular fragment of 80-85 kDa (PMID: 30842511; 34380733). This antibody raised against 1206-1221aa of human L1CAM detects 200-220 kDa full-length L1CAM and 80-kDa cleaved fragment of L1CAM.

Publications

Species	Application	Title
human	IHC	Histopathology; Frequent KRAS mutations in oncocytic papillary renal neoplasm with inverted nuclei.; Authors - Kuo Tong
human	WB	Diagnostics (Basel); Circulatory Astrocyte and Neuronal EVs as Potential Biomarkers of Neurological Dysfunction in HIV-Infected Subjects and Alcohol/Tobacco Users.; Authors - Sunitha Kodidela
human		Anal Chem; Nanosensor-Driven Detection of Neuron-Derived Exosomal Aβ42 with Graphene Electrolyte-Gated Transistor for Alzheimer's Disease Diagnosis; Authors - Jiahao Li
mouse	WB	Res Sq; Curcumin enhances elvitegravir concentration and alleviates oxidative stress and inflammatory response; Authors - Sandip Godse
mouse	WB	Sci Rep; Curcumin enhances elvitegravir concentration and alleviates oxidative stress and inflammatory response; Authors - Sandip Godse