L-VOCC Polyclonal antibody, PBS Only

L-VOCC Polyclonal Antibody for IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 21774-1-PBS

Synonyms

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Validation Data Gallery

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  IHC staining of mouse brain using 21774-1-AP (same clone as 21774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21774-1-AP (L-VOCC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21774-1-AP (same clone as 21774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21774-1-AP (L-VOCC antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 21774-1-AP (same clone as 21774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver using 21774-1-AP (L-VOCC antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 21774-1-AP (same clone as 21774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver using 21774-1-AP (L-VOCC antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 21774-1-AP (same clone as 21774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21774-1-AP (L-VOCC Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 21774-1-AP (same clone as 21774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21774-1-AP (L-VOCC Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 21774-1-AP (same clone as 21774-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L-VOCC antibody (21774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 21774-1-AP (same clone as 21774-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L-VOCC antibody (21774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 21774-1-AP (same clone as 21774-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human L-VOCC (21774-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

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Product Information

21774-1-PBS targets L-VOCC in IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: L-VOCC fusion protein Ag16455
• Full Name: calcium channel, voltage-dependent, L type, alpha 1C subunit
• Calculated Molecular Weight: 2135 aa, 239 kDa
• GenBank Accession Number: BC146846
• Gene Symbol: CACNA1C
• Gene ID (NCBI): 775
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.