L-Plastin Polyclonal antibody

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  WB analysis using 55208-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55208-1-AP (L-Plastin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 55208-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55208-1-AP (L-Plastin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 55208-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 55208-1-AP (L-Plastin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

L-Plastin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 55208-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

LCP1, L PLASTIN, LCP 1, LCP-1, LPL

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, Ramos cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55208-1-AP targets L-Plastin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: lymphocyte cytosolic protein 1 (L-plastin)
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 64-70 kDa
• GenBank Accession Number: NM_002298
• Gene Symbol: L-Plastin
• Gene ID (NCBI): 3936
• RRID: AB_2881288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13796
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LCP1, also known as L-Plastin, is a member of the Plastin gene family. The family members include L-Plastin (LCP1), T-Plastin (PLS3), and I-Plastin (PLS1), which can regulate the dynamic reorganization of the cytoskeleton by binding and cross-linking actin filaments. In lymphocytes, LCP1 can regulate immune responses, especially during inflammation and infection. It acts as a key regulator of T-cell activation by responding to co-stimulatory signals through TCR/CD3 and CD2 or CD28, and regulates the cell-surface expression of IL2RA/CD25 and CD69 (PMID: 17294403).