Kv4.2 Recombinant antibody

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  WB analysis using 85133-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85133-3-RR (KCND2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 85133-3-RR

  DU 145 cells were subjected to SDS PAGE followed by western blot with 85133-3-RR (KCND2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 85133-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85133-3-RR (Kv4.2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 85133-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85133-3-RR (Kv4.2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 85133-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Kv4.2 antibody (85133-3-RR, Clone: 242754F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85133-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85133-3-RR against Human Kv4.2 were performed. The affinity constant is below 1 pM.

Kv4.2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 85133-3-RR

Clone No.242754F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

KCND2, 242754F4, Voltage-gated potassium channel subunit Kv4.2, RK5, Potassium voltage-gated channel subfamily D member 2

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Tested Applications

• Positive WB detected in: HeLa cells, DU 145 cells, A549 cells
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85133-3-RR targets Kv4.2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Kv4.2 fusion protein Ag15879
• Full Name: potassium voltage-gated channel, Shal-related subfamily, member 2
• Calculated Molecular Weight: 630 aa, 71 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC110449
• Gene Symbol: KCND2
• Gene ID (NCBI): 3751
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NZV8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Voltage-gated potassium or Kv channels, specifically those mediating low threshold, rapidly inactivating Ito and IA currents, are known to regulate cardiac and neuronal membrane excitability, respectively (PMID: 12829703). Voltage-gated potassium channel subunit Kv4.2, encoded by the KCND2 gene, belongs to the potassium channel family and D (Shal) subfamily. It is a pore-forming alpha subunit of voltage-gated rapidly inactivating A-type potassium channels. Kv4.2 is highly expressed in the brain (PMID: 10551270). It is a major constituent of A-type potassium currents and a key regulator of neuronal membrane excitability (PMID: 22539834).