Kininogen 1 Polyclonal antibody

Kininogen 1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27477-1-AP

Synonyms

BDK, Fitzgerald factor, HMWK, Ile Ser Bradykinin Bradykinin, Kallidin I Lysyl bradykinin, kininogen 1, KNG, KNG1

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Validation Data Gallery

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  WB analysis using 27477-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27477-1-AP (Kininogen 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27477-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27477-1-AP (Kininogen 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 27477-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27477-1-AP (Kininogen 1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 27477-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27477-1-AP (Kininogen 1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A2780 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27477-1-AP targets Kininogen 1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Kininogen 1 fusion protein Ag26467
• Full Name: kininogen 1
• Calculated Molecular Weight: 644 aa, 72 kDa
• Observed Molecular Weight: 70-72 kDa
• GenBank Accession Number: BC060039
• Gene Symbol: Kininogen 1
• Gene ID (NCBI): 3827
• RRID: AB_2880882
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This gene is translated into High-molecular weight kininogen (HMWK) and low-molecular weight kininogen (LMWK) after alternative splicing. HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.