Kindlin 3 Polyclonal antibody, PBS Only

Kindlin 3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18131-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of Jurkat using 18131-1-AP (same clone as 18131-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18131-1-AP (same clone as 18131-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18131-1-AP (Kindlin 3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18131-1-AP (same clone as 18131-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18131-1-AP (Kindlin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 18131-1-AP (same clone as 18131-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 18131-1-AP (same clone as 18131-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 18131-1-AP (same clone as 18131-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 18131-1-AP (same clone as 18131-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 18131-1-AP (same clone as 18131-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 18131-1-AP (same clone as 18131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 18131-1-AP (FERMT3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 18131-1-AP (same clone as 18131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 18131-1-AP (FERMT3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 18131-1-AP (same clone as 18131-1-PBS)

  Immunofluorescent analysis of HeLa cells using 18131-1-AP (FERMT3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

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Product Information

18131-1-PBS targets Kindlin 3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Kindlin 3 fusion protein Ag12713
• Full Name: fermitin family homolog 3 (Drosophila)
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 72-76 kDa
• GenBank Accession Number: BC004347
• Gene Symbol: Kindlin 3/FERMT3
• Gene ID (NCBI): 83706
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Kindlin-3 (FERMT-3) is known to be central in hemostasis and thrombosis control and its deficiency disrupts platelet aggregation and causes Leukocyte Adhesion Deficiency disease. Point mutations in the kindlin-3 gene have been identified in humans with a rare inherited Integrin Activation efficiency Disease (IADD), also designated as Leukocyte Adhesion Deficiency syndrome (LAD-III or LADI variant). Kindlin-3 expression is restricted to cells of hematopoietic origin.