Keratin 81 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Keratin 81 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11342-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 11342-1-AP (same clone as 11342-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11342-1-AP (same clone as 11342-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 11342-1-AP (same clone as 11342-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

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  WB analysis of human spleen using 11342-1-AP (same clone as 11342-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11342-1-AP (same clone as 11342-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 11342-1-AP (same clone as 11342-1-PBS)

  IP result of anti-KRT81 (IP:11342-1-AP, 4ug; Detection:11342-1-AP 1:300) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11342-1-AP (same clone as 11342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11342-1-AP (KRT81 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11342-1-AP (same clone as 11342-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KRT81 antibody (11342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

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Product Information

11342-1-PBS targets Keratin 81 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Keratin 81 fusion protein Ag1890
• Full Name: keratin 81
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC021241
• Gene Symbol: KRT81
• Gene ID (NCBI): 3887
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT81 is a type II keratin.