Cytokeratin 77 Recombinant antibody

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  WB analysis of HeLa using 83415-3-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 83415-3-RR (KRT77 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83415-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83415-3-RR against Human KRT77 were performed. The affinity constant is 0.209 nM.

Cytokeratin 77 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 83415-3-RR

Clone No.240490A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

KRT77, 240490A11, CK-1B, Cytokeratin,Keratin, Cytokeratin-1B

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83415-3-RR targets Cytokeratin 77 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cytokeratin 77 fusion protein Ag21942
• Full Name: keratin 77
• Calculated Molecular Weight: 578 aa, 62 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC122558
• Gene Symbol: KRT77
• Gene ID (NCBI): 374454
• RRID: AB_3671064
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q7Z794
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins.The KRT77 gene is a type II epithelial cell α-keratin gene family member that is expressed in the skin and eccrine sweat glands. KRT77 has N- and C-terminal domains and a central rod domain consisting of 4 alpha-helical regions separated by 3 nonhelical linker sequences.