KYNU Polyclonal antibody

• Featured Product
• KD/KO Validated

KYNU Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 11796-1-AP

Synonyms

L-kynurenine hydrolase, L kynurenine hydrolase, Kynureninase, EC:3.7.1.3

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Validation Data Gallery

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  WB analysis of HeLa using 11796-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11796-1-AP (KYNU antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11796-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11796-1-AP (KYNU antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 11796-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11796-1-AP (KYNU antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11796-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11796-1-AP (KYNU antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11796-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KYNU antibody (11796-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11796-1-AP

  Immunofluorescent analysis of HepG2 cells, using KYNU antibody 11796-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, HepG2 cells, rat liver tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

11796-1-AP targets KYNU in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KYNU fusion protein Ag2365
• Full Name: kynureninase (L-kynurenine hydrolase)
• Calculated Molecular Weight: 465aa,52 kDa; 307aa,35 kDa
• Observed Molecular Weight: 45-52 kDa
• GenBank Accession Number: BC000879
• Gene Symbol: KYNU
• Gene ID (NCBI): 8942
• RRID: AB_2133050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KYNU(kynureninase) is also named as L-kynurenine hydrolase and belongs to the kynureninase family. It is a pyridoxal-5'-phosphate-(pyridoxal-P)-depe11dent enzyme that catalyses the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively(PMID:8706755). In SDS-PAGE, it can detect a 48 kDa band lower than the preidicted molecular weight of 52 kDa due to limited proteolysis of the enzyme which might occur during handling of the protein(PMID:8706755). This protein is a 95-kD kD homodimer predominantly located in the cytoplasm(PMID:9180257). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	IF	J Hazard Mater; Particulate matter-induced metabolic recoding of epigenetics in macrophages drives pathogenesis of chronic obstructive pulmonary disease; Authors - Myungkyung Noh
human	WB	Virol Sin; Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection.; Authors - Yingying Song
rat	WB	Chem Res Toxicol; Tryptophan Pathway-Targeted Metabolomics Study on the Mechanism and Intervention of Cisplatin-Induced Acute Kidney Injury in Rats.; Authors - Bei Tan
human	WB	Clin Exp Dermatol; Downregulation of kynureninase restrains cutaneous squamous cell carcinoma proliferation and represses the PI3K/AKT pathway.; Authors - C Ci ; KD Validated
human	WB	Adv Sci (Weinh); Tryptophan Metabolism Acts as a New Anti-Ferroptotic Pathway to Mediate Tumor Growth; Authors - Dong Liu ; KO Validated
human	WB,IHC	Oncogene; Glutathione peroxidase 2 knockdown suppresses gastric cancer progression and metastasis via regulation of kynurenine metabolism; Authors - Handong Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hala (Verified Customer) (03-29-2022)

it works good in BSA 5% dissolved in TBST

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: hela cells

KYNU Antibody Western Blot validation (1/1000 dilution) in hela cells (Cat no:11796-1-AP)