KRT80 Polyclonal antibody

• Featured Product
• KD/KO Validated

KRT80 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 16835-1-AP

Synonyms

KB20, K80, Cytokeratin-80, Cytokeratin 80, CK-80

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Validation Data Gallery

• 

  WB analysis of NIH/3T3 using 16835-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16835-1-AP (KRT80 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 16835-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16835-1-AP (KRT80 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 16835-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16835-1-AP (KRT80 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 16835-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16835-1-AP (KRT80 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 16835-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 16835-1-AP (KRT80 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 16835-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 16835-1-AP (KRT80 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16835-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 16835-1-AP (KRT80 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16835-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 16835-1-AP (KRT80 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16835-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16835-1-AP (KRT80 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16835-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16835-1-AP (KRT80 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, human skeletal muscle tissue
• Positive IHC detected in: human skin cancer tissue, human bowen disease, human colon cancer, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3600
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 6 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Product Information

16835-1-AP targets KRT80 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KRT80 fusion protein Ag10498
• Full Name: keratin 80
• Calculated Molecular Weight: 422 aa, 47 kDa
• Observed Molecular Weight: 47 kDa, 50 kDa
• GenBank Accession Number: BC065180
• Gene Symbol: KRT80
• Gene ID (NCBI): 144501
• RRID: AB_1851273
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KRT80 is a human IF type II epithelial keratin gene involved in the formation of IF heterodimers in various epithelial cells. KRT80 is overexpressed in many neoplasms and plays an essential role in promoting cell proliferation, migration and invasiveness, and is associated with poor prognosis in cancer patients. KRT80 has three isoforms with predicted MW of 50 kDa, 47 kDa, and 54 kDa.

Publications

Species	Application	Title
human	WB	Mol Cell; Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine.; Authors - Daniel Arango
human	IHC	Cell Discov; Single-cell atlas of keratoconus corneas revealed aberrant transcriptional signatures and implicated mechanical stretch as a trigger for keratoconus pathogenesis.; Authors - Shengqian Dou
human	IF	Int J Mol Sci; Keratin Profiling by Single-Cell RNA-Sequencing Identifies Human Prostate Stem Cell Lineage Hierarchy and Cancer Stem-Like Cells.; Authors - Wen-Yang Hu
human	WB,IF	Cell Death Dis; Keratin 80 promotes migration and invasion of colorectal carcinoma by interacting with PRKDC via activating the AKT pathway.; Authors - Changcan Li
human	WB	Cell Cycle; MiR-4268 suppresses gastric cancer genesis through inhibiting keratin 80.; Authors - Fan Zhang ; KD Validated
human	WB,IHC	Cancer Biol Ther; The facilitating effects of KRT80 on chemoresistance, lipogenesis, and invasion of esophageal cancer; Authors - Wen-Jing Yun ; KD Validated