Cytokeratin 8 Polyclonal antibody

• Featured Product
• KD/KO Validated

Cytokeratin 8 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 10384-1-AP

Synonyms

KRT8, Cytokeratin-8, Cytokeratin,Keratin, CK-8, CK8

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Validation Data Gallery

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  WB analysis using 10384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10384-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10384-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10384-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10384-1-AP

  IP result of anti-Cytokeratin 8 (IP:10384-1-AP, 4ug; Detection:10384-1-AP 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human tonsillitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded normal colon slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human breast cancer using 10384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10384-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 8 antibody (10384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 10384-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 8 antibody (10384-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

• 

  FC experiment of MCF-7 using 10384-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Cytokeratin 8 Polyclonal antibody (10384-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, HaCaT cells, COLO 320 cells, A431 cells, A549 cells, MCF-7 cells, mouse skin tissue, rat skin tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human tonsillitis tissue, human colon tissue, human breast cancer tissue, human liver tissue, human appendicitis tissue, human renal cell carcinoma tissue, human bowen disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue
• Positive IF/ICC detected in: A431 cells, HeLa cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:40000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Product Information

10384-1-AP targets Cytokeratin 8 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, goat, dog
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 8 fusion protein Ag0488
• Full Name: keratin 8
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC000654
• Gene Symbol: Cytokeratin 8
• Gene ID (NCBI): 3856
• RRID: AB_10638912
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytokeratin 8 (CK8) is a protein that belongs to the cytokeratin family, which is a group of structural proteins found in the intracytoplasmic cytoskeleton of epithelial cells. CK8 forms intermediate filaments within the cytoplasm of simple epithelial cells, typically as a dimer with cytokeratin 18 (CK18). These filaments provide a stabilizing framework that helps determine cell shape and enables cells to cope with mechanical stress. CK8/CK18 filaments also serve as a mesh of "paths" for signaling molecules, metabolites, and pathogens to travel within the cell in an organized manner. CK8 is expressed in various epithelial tissues and is particularly associated with glandular and simple epithelia. Its expression is also a significant marker in several types of cancer, including lung and esophageal cancer, where it can indicate tumor progression and poor prognosis.lar functions such as mechanical support, stem cell-fate control, and cytoprotective response.

Publications

Species	Application	Title
mouse	IF	Dev Cell; An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migration; Authors - Shifaan Thowfeequ
human	WB,IF,IP	J Cell Sci; SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network; Authors - Archan Chakraborty ; KD Validated
mouse	WB,IF	Front Cell Dev Biol; ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2.; Authors - Shuai Yu
human	WB,IF	J Cell Sci; SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network.; Authors - Archan Chakraborty ; KD Validated
human, goat, dog, pig	IHC	Anat Rec (Hoboken); Comparative study of the external auditory canal in humans and large mammals.; Authors - Jun Wang
human	WB,IF	Oncotarget; Alternative promotion and suppression of metastasis by JNK2 governed by its phosphorylation.; Authors - Sike Hu

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