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- KD/KO Validated
Cytokeratin 8 Polyclonal antibody
Cytokeratin 8 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10384-1-AP
Validation Data Gallery
|Positive WB detected in
|HeLa cells, HaCaT cells, COLO 320 cells, A431 cells, A549 cells, MCF-7 cells, mouse skin tissue, rat skin tissue
|Positive IP detected in
|Positive IHC detected in
|human tonsillitis tissue, human colon tissue, human appendicitis tissue, human breast cancer tissue, human renal cell carcinoma tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|A431 cells, HepG2 cells, human breast cancer tissue, HeLa cells
|Positive FC detected in
|HepG2 cells, MCF-7 cells
|Western Blot (WB)
|WB : 1:5000-1:40000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:1000-1:4000
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10384-1-AP targets Cytokeratin 8 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, dog, goat, pig
|Host / Isotype
|Rabbit / IgG
|Cytokeratin 8 fusion protein Ag0488
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.
|Product Specific Protocols
|WB protocol for Cytokeratin 8 antibody 10384-1-AP
|IHC protocol for Cytokeratin 8 antibody 10384-1-AP
|IF protocol for Cytokeratin 8 antibody 10384-1-AP
|IP protocol for Cytokeratin 8 antibody 10384-1-AP
|Click here to view our Standard Protocols
J Cell Sci
SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network
Front Cell Dev Biol
ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2.
J Cell Sci
SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network.
Anat Rec (Hoboken)
Comparative study of the external auditory canal in humans and large mammals.
Alternative promotion and suppression of metastasis by JNK2 governed by its phosphorylation.
The helix-loop-helix transcriptional regulator Id4 is required for terminal differentiation of luminal epithelial cells in the prostate.