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  • KD/KO Validated

Cytokeratin 8 Polyclonal antibody

Cytokeratin 8 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10384-1-AP

Synonyms

CARD2, Cell and organelle markers, CK 8, CK8, CYK8, Cytokeratin 8, Cytoskeleton Marker, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8



Tested Applications

Positive WB detected inHeLa cells, HaCaT cells, COLO 320 cells, A431 cells, A549 cells, MCF-7 cells, mouse skin tissue, rat skin tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue, human colon tissue, human appendicitis tissue, human breast cancer tissue, human renal cell carcinoma tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, HepG2 cells, human breast cancer tissue, HeLa cells
Positive FC detected inHepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:40000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10384-1-AP targets Cytokeratin 8 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, dog, goat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 8 fusion protein Ag0488
Full Name keratin 8
Calculated molecular weight 54 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC000654
Gene symbol KRT8
Gene ID (NCBI) 3856
RRIDAB_10638912
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 8 antibody 10384-1-APDownload protocol
IHC protocol for Cytokeratin 8 antibody 10384-1-APDownload protocol
IF protocol for Cytokeratin 8 antibody 10384-1-APDownload protocol
IP protocol for Cytokeratin 8 antibody 10384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,IP

J Cell Sci

SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network

Authors - Archan Chakraborty
  • KD Validated
mouseWB,IF

Front Cell Dev Biol

ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2.

Authors - Shuai Yu
humanWB,IF

J Cell Sci

SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network.

Authors - Archan Chakraborty
  • KD Validated
human, goat, dog, pigIHC

Anat Rec (Hoboken)

Comparative study of the external auditory canal in humans and large mammals.

Authors - Jun Wang
humanWB,IF

Oncotarget

Alternative promotion and suppression of metastasis by JNK2 governed by its phosphorylation.

Authors - Sike Hu
mouseIHC

Oncoscience

The helix-loop-helix transcriptional regulator Id4 is required for terminal differentiation of luminal epithelial cells in the prostate.

Authors - Dhanushka Hewa Bostanthirige