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Cytokeratin 6B Polyclonal antibody
Cytokeratin 6B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 17391-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse skin tissue, A431 cells, rat skin tissue |
Positive IHC detected in | human lung cancer tissue, human cervical cancer tissue, human breast cancer tissue, human tonsil tissue, human oesophagus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1600-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 1 publications below |
IHC | See 1 publications below |
Product Information
17391-1-AP targets Cytokeratin 6B in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Cytokeratin 6B fusion protein Ag11288 |
Full Name | keratin 6B |
Calculated Molecular Weight | 564 aa, 60 kDa |
Observed Molecular Weight | 60 kDa |
GenBank Accession Number | BC034535 |
Gene Symbol | Cytokeratin 6B |
Gene ID (NCBI) | 3854 |
RRID | AB_2134318 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as a marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B, and 6C. They share more than 99% identical DNA sequence.
Protocols
Product Specific Protocols | |
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WB protocol for Cytokeratin 6B antibody 17391-1-AP | Download protocol |
IHC protocol for Cytokeratin 6B antibody 17391-1-AP | Download protocol |
IF protocol for Cytokeratin 6B antibody 17391-1-AP | Download protocol |
FC protocol for Cytokeratin 6B antibody 17391-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Invest Dermatol Frizzled6 deficiency disrupts the differentiation process of nail development. | ||
J Transl Med Bladder cancer-derived exosomal KRT6B promotes invasion and metastasis by inducing EMT and regulating the immune microenvironment.
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