Cytokeratin 6B Polyclonal antibody

• Featured Product
• KD/KO Validated

Cytokeratin 6B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17391-1-AP

Synonyms

KRT6B, Cytokeratin-6B, Cytokeratin,Keratin, CK-6B, CK6B

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Validation Data Gallery

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  WB analysis of mouse skin using 17391-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17391-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skin using 17391-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 17391-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 17391-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsil using 17391-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human tonsil using 17391-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human oesophagus using 17391-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 17391-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 17391-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17391-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus using 17391-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 17391-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 17391-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 6B antibody (17391-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 17391-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 6B (17391-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse skin tissue, A431 cells, rat skin tissue
• Positive IHC detected in: human lung cancer tissue, human cervical cancer tissue, human breast cancer tissue, human tonsil tissue, human oesophagus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1600-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

17391-1-AP targets Cytokeratin 6B in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 6B fusion protein Ag11288
• Full Name: keratin 6B
• Calculated Molecular Weight: 564 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC034535
• Gene Symbol: Cytokeratin 6B
• Gene ID (NCBI): 3854
• RRID: AB_2134318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as a marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B, and 6C. They share more than 99% identical DNA sequence.

Publications

Species	Application	Title
mouse	IHC	J Invest Dermatol; Frizzled6 deficiency disrupts the differentiation process of nail development.; Authors - Cui Chang-Yi CY
human	WB	J Transl Med; Bladder cancer-derived exosomal KRT6B promotes invasion and metastasis by inducing EMT and regulating the immune microenvironment.; Authors - Qiang Song ; KD Validated

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C