Cytokeratin 3 Polyclonal antibody

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  WB analysis using 16850-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16850-1-AP (Cytokeratin 3-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Cytokeratin 3 Polyclonal Antibody for WB, ELISA

Cat No. 16850-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Cytokeratin 3-specific, KRT3, keratin 3, Cytokeratin-3, CK-3

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16850-1-AP targets Cytokeratin 3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: keratin 3
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: NM_057088
• Gene Symbol: Cytokeratin 3
• Gene ID (NCBI): 3850
• RRID: AB_3669258
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P12035
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a member of the type II cytokeratin subfamily, KRT3 is expressed together with KRT12 during 'corneal-type' differentiation.