Cytokeratin 20 Polyclonal antibody

Cytokeratin 20 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 17329-1-AP

Synonyms

KRT20, keratin 20, K20, Cytokeratin-20, CK-20

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Validation Data Gallery

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  WB analysis using 17329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17329-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17329-1-AP (Cytokeratin 20 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Rabbit colon using 17329-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Rabbit colon tissue using Cytokeratin 20 antibody (17329-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of Rabbit colon using 17329-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Rabbit colon tissue using Cytokeratin 20 antibody (17329-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of Caco-2 using 17329-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Cytokeratin 20 antibody (17329-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 17329-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HT-29 using 17329-1-AP

  1X10^6 HT-29 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 20 (17329-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, HT-29 cells
• Positive IHC detected in: human colon tissue, human appendicitis tissue, human colon cancer tissue, human stomach cancer tissue, human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: Rabbit colon tissue
• Positive IF/ICC detected in: Caco-2 cells, HeLa cells
• Positive FC (Intra) detected in: HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Product Information

17329-1-AP targets Cytokeratin 20 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

• Tested Reactivity: human, mouse, rat, rabbit
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 20 fusion protein Ag11308
• Full Name: keratin 20
• Calculated Molecular Weight: 424 aa, 48 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC031559
• Gene Symbol: Cytokeratin 20
• Gene ID (NCBI): 54474
• RRID: AB_2133592
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

Publications

Species	Application	Title
mouse	IF	Cell Res; Gut microbiota drives macrophage-dependent self-renewal of intestinal stem cells via niche enteric serotonergic neurons.; Authors - Pingping Zhu
mouse	WB	Nat Cell Biol; LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.; Authors - Pingping Zhu
		Neuron; 5-hydroxytryptamine produced by enteric serotonergic neurons initiates colorectal cancer stem cell self-renewal and tumorigenesis.; Authors - Pingping Zhu
human	IF	Biomaterials; A microengineered collagen scaffold for generating a polarized crypt-villus architecture of human small intestinal epithelium.; Authors - Yuli Wang
mouse	IF	Cancer Lett; LGR4 cooperates with PrPc to endow the stemness of colorectal cancer stem cells contributing to tumorigenesis and liver metastasis.; Authors - Qi Cheng
mouse	IHC	Nutrients; Propolis Has an Anticancer Effect on Early Stage Colorectal Cancer by Affecting Epithelial Differentiation and Gut Immunity in the Tumor Microenvironment; Authors - Ming-Hung Shen

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