Validation Data Gallery
|Positive WB detected in
|COLO 320 cells, HeLa cells, MCF-7 cells, HT-29 cells
|Positive IHC detected in
|human colon tissue, human appendicitis tissue, human stomach cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HT-29 cells, HeLa cells, Rabbit colon tissue
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:16000
|IHC : 1:1000-1:4000
|IF : 1:1000-1:4000
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
17329-1-AP targets Cytokeratin 20 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.
|human, mouse, rat, rabbit
|Host / Isotype
|Rabbit / IgG
|Cytokeratin 20 fusion protein Ag11308
|Calculated molecular weight
|424 aa, 48 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.
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