Cytokeratin 2e Polyclonal antibody

Cytokeratin 2e Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21725-1-AP

Synonyms

CK 2e, Cytokeratin 2e, Epithelial keratin 2e, K2e, keratin 2, Keratin 2 epidermis, Keratin 2e, KRT2, KRT2A, KRT2E, KRTE, Type II keratin Kb2

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Validation Data Gallery

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  WB analysis using 21725-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21725-1-AP (KRT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 21725-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21725-1-AP (KRT2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 21725-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21725-1-AP (KRT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 21725-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21725-1-AP (KRT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 21725-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 21725-1-AP (KRT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 21725-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 21725-1-AP (KRT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, mouse skin tissue, rat skin tissue.
• Positive IHC detected in: human cervical cancer tissue, human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

21725-1-AP targets Cytokeratin 2e in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 2e fusion protein Ag16384
• Full Name: keratin 2
• Calculated Molecular Weight: 639 aa, 66 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC096294
• Gene Symbol: KRT2
• Gene ID (NCBI): 3849
• RRID: AB_2878914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 2 is a type II cytokeratin. It is found largely in the upper spinous layer of epidermal keratinocytes, and also in other regions, notably the epidermis covering the knee, thigh, and groin.

Publications

Species	Application	Title
human	IF	Cont Lens Anterior Eye; The biomechanical proteins different between low myopic corneas and moderate to high myopic corneas in human; Authors - Wenjing Wu