Cytokeratin 17 Polyclonal antibody

Cytokeratin 17 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 18502-1-AP

Synonyms

KRT17, Keratin-17, keratin 17, Cytokeratin-17, Cytokeratin,Keratin

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Validation Data Gallery

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  WB analysis of HeLa using 18502-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 18502-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18502-1-AP

  IP result of anti-Cytokeratin 17 (IP:18502-1-AP, 4ug; Detection:18502-1-AP 1:600) with HeLa cells lysate 1600ug.

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  IHC staining of human cervical cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18502-1-AP (Cytokeratin 17 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18502-1-AP (Cytokeratin 17 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 18502-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 17 antibody (18502-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 18502-1-AP

  Immunofluorescent analysis of HeLa cells using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue, human lung cancer tissue, human colon cancer tissue, human pancreas cancer tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• ELISA: See 1 publications below

Product Information

18502-1-AP targets Cytokeratin 17 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 17 fusion protein Ag13351
• Full Name: keratin 17
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC011901
• Gene Symbol: Cytokeratin 17
• Gene ID (NCBI): 3872
• RRID: AB_10644296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filaments that make up the cytoskeleton. Type I keratins are a group of acidic intermediate filament proteins and type II keratins are the basic or neutral counterparts. Keratin 17 is encoded by the KRT17 gene and is a type I cytokeratin found in nail beds, hair follicles, sebaceous glands, and epidermal appendages.

 What is the molecular weight of cytokeratin 17?

The molecular weight of cytokeratin 17 is approximately 49 kDa.

 What is the function of cytokeratin 17?

Cytokeratin 17 plays a role in the formation and maintenance of epidermal appendages, especially in determining the shape and orientation of hair. It is required for the maintenance of the anagen (growth) state of hair follicles by modulating the function of TNF-alpha for hair cycling and is also involved in tissue repair (PMID: 16702408).

 What is the cellular localization of cytokeratin 17?

Cytokeratin 17 expression is located in the cytoplasm.

 What is the tissue specificity of cytokeratin 17?

Cytokeratin 17 expression is found in the outer root sheath and medulla regions of the hair follicle, particularly in the eyebrow, beard, nail bed epithelium, basal cells of the oral epithelium, and sweat and mammary glands (PMID: 2451629). Expression can also be found in epithelial layers of the prostate and urinary bladder and cambial cells of the sebaceous gland at the protein level.

 What is the role of cytokeratin 17 in disease?

Mutations in the KRT17 gene are a cause of Jackson-Lawler type pachyonychia congenita type 2 (PC2); an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy (PMID: 7539673). Defects in KRT17 also lead to steatocystoma multiplex (SM) (PMID: 9767294). Cytokeratin 17 acts as an autoantigen in the immunopathogenesis of psoriasis (PMID: 23722817).

Publications

Species	Application	Title
goat	IF	J Anim Sci Biotechnol; Establishment of goat mammary organoid cultures modeling the mammary gland development and lactation; Authors - Lei Jia
human,mouse	WB,IHC,IF,ELISA	J Endocr Soc; KRT17 From Keratinocytes With High Glucose Stimulation Inhibit Dermal Fibroblasts Migration Through Integrin α11; Authors - Peng Zhou
human	WB,IHC	Research (Wash D C); Aggregation-Induced Emission-Active Photosensitizer-Mediated Photodynamic Therapy for Anti-Psoriasis; Authors - Ping Zhu
human	IF	J Inflamm Res; CORM-3 Inhibits the Inflammatory Response of Human Periodontal Ligament Fibroblasts Stimulated by LPS and High Glucose; Authors - Haoyang Tian