Cytokeratin 13 Polyclonal antibody

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• KD/KO Validated

Cytokeratin 13 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 10164-2-AP

Synonyms

KRT13, Cytokeratin-13, Cytokeratin,Keratin, ck17, CK-13

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Validation Data Gallery

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  WB analysis of A431 using 10164-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10164-2-AP

  WB result of Cytokeratin 13 antibody (10164-2-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 13 transfected A431 cells.

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  WB analysis of mouse skin using 10164-2-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10164-2-AP (Cytokeratin 13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 10164-2-AP

  IP result of anti-Cytokeratin 13 (IP:10164-2-AP, 4ug; Detection:10164-2-AP 1:1000) with A431 cells lysate 2400ug.

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  IHC staining of human cervical cancer using 10164-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 10164-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human oesophagus using 10164-2-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human oesophagus using 10164-2-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HaCaT using 10164-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 13 antibody (10164-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 10164-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 13 antibody (10164-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A431 using 10164-2-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 13 (10164-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, mouse skin tissue
• Positive IP detected in: A431 cells
• Positive IHC detected in: human cervical cancer tissue, human oesophagus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HaCaT cells, A431 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 11 publications below

Product Information

10164-2-AP targets Cytokeratin 13 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 13 fusion protein Ag0217
• Full Name: keratin 13
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC002661
• Gene Symbol: Cytokeratin 13
• Gene ID (NCBI): 3860
• RRID: AB_2134679
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.

Publications

Species	Application	Title
mouse	IF	Theranostics; Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p; Authors - Soo Hyun Kang
human	IF	Elife; Chloride channels regulate differentiation and barrier functions of the mammalian airway.; Authors - Mu He
human	WB	Cell Commun Signal; Liprin-α1 modulates cancer cell signaling by transmembrane protein CD82 in adhesive membrane domains linked to cytoskeleton.; Authors - Henna Pehkonen
human	IF	Am J Respir Cell Mol Biol; Do airway epithelium air-liquid cultures represent the in vivo airway epithelium transcriptome?; Authors - Dvorak Anna A
human	IF	Stem Cells Int; Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.; Authors - Cong Li
human	IHC	Mol Vis; Expression analysis of human pterygium shows a predominance of conjunctival and limbal markers and genes associated with cell migration.; Authors - Jaworski C J CJ