Cytokeratin 10 Polyclonal antibody

Cytokeratin 10 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

17

Reviews (1)

Cat no : 18343-1-AP

Synonyms

KRT10, K10, Cytokeratin-10, Cytokeratin,Keratin, CK-10

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Validation Data Gallery

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  WB analysis using 18343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 18343-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of A431 using 18343-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 10 antibody (18343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of A431 using 18343-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 10 (18343-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse skin tissue, A431 cells, rat skin tissue
• Positive IHC detected in: human cervical cancer tissue, mouse skin tissue, rat skin tissue, human lung cancer tissue, human breast cancer tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 10 publications below

Product Information

18343-1-AP targets Cytokeratin 10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 10 fusion protein Ag13136
• Full Name: keratin 10
• Calculated Molecular Weight: 584 aa, 59 kDa
• Observed Molecular Weight: 50-59 kDa
• GenBank Accession Number: BC034697
• Gene Symbol: Cytokeratin 10
• Gene ID (NCBI): 3858
• RRID: AB_10863654
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia.

Publications

Species	Application	Title
rat	IF	Cell Mol Biol Lett; The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum synthesis; Authors - Tao Yang
mouse	IF	Aging Cell; Nociceptive transient receptor potential canonical 7 (TRPC7) mediates aging-associated tumorigenesis induced by ultraviolet B.; Authors - Wen-Li Hsu
mouse	IHC	Life Sci; The potential of Diosgenin in treating psoriasis: Studies from HaCaT keratinocytes and imiquimod-induced murine model.; Authors - Shidi Wu
human	IF	mBio; Commensal-Related Changes in the Epidermal Barrier Function Lead to Alterations in the Benzo[a]Pyrene Metabolite Profile and Its Distribution in 3D Skin.; Authors - Lisa Lemoine
human	IF	Eur Surg Res; A novel combination of keratinocyte and autologous microskin grafting to repair full thickness skin loss; Authors - Kai Yin
mouse	IF	Int J Biol Macromol; Skin-adaptive film dressing with smart-release of growth factors accelerated diabetic wound healing; Authors - Yingzheng Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joshua (Verified Customer) (07-27-2019)

Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Bright staining with minimal background.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Keratinocytes

Cytokeratin 10 Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:18343-1-AP)