KRAS-2A-specific Polyclonal antibody

KRAS-2A-specific Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 16156-1-AP

Synonyms

KRAS-2A, KRAS, KRAS4A, KRAS4B, pan RAS



Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

16156-1-AP targets KRAS-2A-specific in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21-24 kDa
GenBank Accession NumberNM_033360
Gene Symbol KRAS
Gene ID (NCBI) 3845
RRIDAB_2234477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAS (derived from 'RAt Sarcoma' virus) gene family includes KRAS, HRAS, and NRAS. These genes encode very similar 21 kDa proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 2A and 2B. This antibody is specific for KRAS-2A, which also named as KRAS-4A .

Protocols

Product Specific Protocols
WB protocol for KRAS-2A-specific antibody 16156-1-APDownload protocol
IF protocol for KRAS-2A-specific antibody 16156-1-APDownload protocol
FC protocol for KRAS-2A-specific antibody 16156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Chem Biol

Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System.

Authors - Sascha Röth
humanWB

Sci Signal

Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies.

Authors - Andrew M Waters
humanWB

Life Sci Alliance

A new ferrocene derivative blocks K-Ras localization and function by oxidative modification at His95

Authors - Kristen M Rehl