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  • Featured Product
  • KD/KO Validated

KRAS-2B Polyclonal antibody

KRAS-2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16155-1-AP

Synonyms

KRAS-2B-specific, KRAS, KRAS4A, KRAS4B, pan RAS

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KRAS-2B Polyclonal antibody (16155-1-AP)

    WB analysis of HeLa using 16155-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KRAS-2B-specific Polyclonal antibody (16155-1-AP)

    WB analysis using 16155-1-AP

    Recombinant proteins(ag27444 (6*His tag-KRAS-2A), ag21645(6*His tag-KRAS-2B), ag29746(6*His tag-NRAS), ag23265(GST-HRAS))were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using KRAS-2B-specific Polyclonal antibody (16155-1-AP)

    WB analysis of HEK-293 using 16155-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using KRAS-2B-specific Polyclonal antibody (16155-1-AP)

    WB analysis of mouse brain using 16155-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using KRAS-2B-specific Polyclonal antibody (16155-1-AP)

    WB analysis of MCF-7 using 16155-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KRAS-2B Polyclonal antibody (16155-1-AP)

    IHC staining of human colon cancer using 16155-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KRAS-2B Polyclonal antibody (16155-1-AP)

    IHC staining of human colon cancer using 16155-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KRAS-2B Polyclonal antibody (16155-1-AP)

    IF Staining of HeLa using 16155-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KRAS-2B-specific antibody (16155-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, mouse brain tissue, Recombinant protein
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16155-1-AP targets KRAS-2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberNM_004985
Gene Symbol KRAS
Gene ID (NCBI) 3845
RRIDAB_2134119
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAS gene family encodes very similar 21KD proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 2A and 2B. This antibody is specific for KRAS-2B.

Protocols

Product Specific Protocols
WB protocol for KRAS-2B antibody 16155-1-APDownload protocol
IHC protocol for KRAS-2B antibody 16155-1-APDownload protocol
IF protocol for KRAS-2B antibody 16155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Nat Biotechnol

Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors.

Authors - Christopher W Davies
View Article
humanFC

Nat Biomed Eng

Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting.

Authors - Mahmoud Labib
View Article
humanWB

Cancer Res

Targeting eIF4A-Dependent Translation of KRAS Signaling Molecules.

Authors - Kamini Singh
View Article
humanWB

Cell Chem Biol

Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System.

Authors - Sascha Röth
View Article
humanWB

Sci Signal

Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies.

Authors - Andrew M Waters
View Article
humanWB

Life Sci Alliance

A new ferrocene derivative blocks K-Ras localization and function by oxidative modification at His95

Authors - Kristen M Rehl
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Christopher (Verified Customer) (12-14-2018)

Antibody is very specific but also quite weak. Maybe you can increase the primary antibody dilution to 1:500 to increase signal. There is no unspecific recognition of H-Ras or N-Ras (see picture). Also endogenous Ras only exhibits one band and not two which is a hint for the recognition of endogenous N-Ras next to K-Ras.In immunofluorescence experiments antibody works awesome at a dilution of 1:100 for pancreatic cancer cell lines. In total I'm very satisfied, but you have to use more antibody than written in the data sheet.

  • Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: pancreatic cancer cells (PANC-1) and HEK293 overexpressing EGFP-tagged K-Ras, H-Ras and N-Ras
KRAS-2B Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:1000 dilution) in pancreatic cancer cells (PANC-1) and HEK293 overexpressing EGFP-tagged K-Ras, H-Ras and N-Ras (Cat no:16155-1-AP)