KRAS Polyclonal antibody

• Featured Product
• KD/KO Validated

KRAS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

134

Reviews (5)

Cat no : 12063-1-AP

Synonyms

KRAS4A, KRAS4B, pan RAS, GTPase KRas, EC:3.6.5.2

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Validation Data Gallery

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  WB analysis using 12063-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12063-1-AP

  WB result of KRAS antibody (12063-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KRAS transfected HEK-293 cells.

• 

  WB analysis using 12063-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12063-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12063-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12063-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12063-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 12063-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 12063-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12063-1-AP

  IP result of anti-KRAS (IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:10000) with HeLa cells lysate 1280 ug.

• 

  IHC staining of human lung cancer using 12063-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12063-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12063-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12063-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12063-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12063-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12063-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KRAS antibody (12063-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 12063-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KRAS (12063-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human brain tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, A549 cells, rat liver tissue, HeLa cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue, human kidney tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 14 publications below
• WB: See 117 publications below
• IHC: See 19 publications below
• IF: See 10 publications below
• IP: See 1 publications below

Product Information

12063-1-AP targets KRAS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KRAS fusion protein Ag2700
• Full Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• Calculated Molecular Weight: 188 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC013572
• Gene Symbol: KRAS
• Gene ID (NCBI): 3845
• RRID: AB_878040
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.

Publications

Species	Application	Title
human	WB	Nat Biotechnol; Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors.; Authors - Christopher W Davies
human	WB	Cancer Discov; LZTR1 mutation mediates oncogenesis through stabilization of EGFR and AXL; Authors - Aram Ko
mouse	WB,IHC	Cell Res; In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.; Authors - Zheng Fu
mouse	WB	Cancer Discov; Systematic functional interrogation of rare cancer variants identifies oncogenic alleles.; Authors - Eejung Kim
human	WB	Mol Cell; The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.; Authors - Linyu Sun
human	WB	Mol Cell; Regulation of GTPase function by autophosphorylation.; Authors - Christian W Johnson

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sophy (Verified Customer) (12-22-2022)

I have tried the KRas antibody from a lot of companies and this one from proteintech definitely has been working the best for me.

• Applications: Western Blot

FH

Jonas (Verified Customer) (07-29-2022)

Antibody works as expected, dilution with 1:2000 was perfect and could even be increased to 1:5000; delivery was very fast, would recommend!!

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000

KRAS Antibody Western Blot validation (1:2000 dilution) in (Cat no:12063-1-AP)

FH

Usman Shah (Verified Customer) (11-29-2021)

Enables specific detection of KRAS in WBs.

• Applications: Western Blot

FH

Kishor (Verified Customer) (12-13-2018)

I am very much satisfied with this antibody. I got good results for cells and rat liver tissue protein.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1, and Rat Liver tissues

FH

Christopher (Verified Customer) (12-11-2018)

Antibody is much better than comparable antibodies. Because of weak expression an very low differences between K-Ras/H-Ras/N-Ras it's quite difficult to detect only K-Ras in a specific way. That is also the problem of this antibody. Please look at my attached blot. 1. Fermentas prestained marker2. PANC-1 whole cell lysate3. PANC-1/EGFP-K-Ras4. HEK293/EGFP-K-Ras5. HEK293/EGFP-H-Ras6.HEK293/EGFP-N-Ras7. 20 ng recombinant K-Ras (His-tagged)Unfortunately, K-Ras (12063-1-AP) recognizes also H-Ras and in less amounts N-Ras. Looking at endogenous K-Ras detection, you have to keep in mind, that the upper band is K-Ras and the lower one H-Ras. Because of the fact, that N-Ras is only recognized in very low amounts (probably almost not on endogenous level) this antibody is great detecting endogenous K-Ras in whole cell lysates with some little problems

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: pancreatic cancer cells (PANC-1) and HEK293 overexpressing EGFP-tagged K-Ras, H-Ras and N-Ras

KRAS Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:1000 dilution) in pancreatic cancer cells (PANC-1) and HEK293 overexpressing EGFP-tagged K-Ras, H-Ras and N-Ras (Cat no:12063-1-AP)

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