Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, HEK-293 cells, human brain tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, rat liver tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells, HGC-27 cells, MKN-45 cells, mouse brain tissue |
| Positive IHC detected in | human lung cancer tissue, human colon cancer tissue, human kidney tissue, human pancreas cancer tissue, mouse kidney tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12063-1-AP targets KRAS in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2700 Product name: Recombinant human KRAS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-188 aa of BC013572 Sequence: MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM Predict reactive species |
| Full Name | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
| Calculated Molecular Weight | 188 aa, 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC013572 |
| Gene Symbol | KRAS |
| Gene ID (NCBI) | 3845 |
| RRID | AB_878040 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01116 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for KRAS antibody 12063-1-AP | Download protocol |
| IF protocol for KRAS antibody 12063-1-AP | Download protocol |
| IHC protocol for KRAS antibody 12063-1-AP | Download protocol |
| IP protocol for KRAS antibody 12063-1-AP | Download protocol |
| WB protocol for KRAS antibody 12063-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-KRAS antibody (Cat# 12063-1-AP) has accumulated 221 total citations published in prestigious journals including Cell, Nature Biotechnology, Nature Communications, Cancer Discovery, Molecular Cell, Science Advances, Oncogene, and ACS Nano. Associated research fields encompass oncology (pancreatic, colorectal, lung, breast cancers), KRAS/MAPK signaling pathways, small-molecule inhibitor discovery, CRISPR/RNAi-based gene editing, ferroptosis, CAR-T immunotherapy, nanomedicine, and cellular metabolism.
| Species | Application | Title |
|---|---|---|
J Hematol Oncol Ovatodiolide suppresses colon tumorigenesis and prevents polarization of M2 tumor-associated macrophages through YAP oncogenic pathways. | ||
Nat Biotechnol Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors. | ||
Nat Biotechnol A rapid imaging-based screen for induced-proximity degraders identifies a potent degrader of oncoprotein SKP2 | ||
Gastroenterology A TEAD2-driven endothelial-like program shapes basal-like differentiation and metastasis of pancreatic cancer |
Reviews
What customers say
Users consistently rate this KRAS antibody highly for Western blot, praising its strong signal and reliable detection of endogenous KRAS with minimal background. Recommended dilutions range from 1:1000 to 1:2000, with one user successfully using 1:5000. Several reviewers note it outperforms comparable antibodies from other vendors. The antibody has been validated in human cell lines (AGS, PANC-1, HEK293, H69, HUCCT-1) and rat liver tissue. One reviewer noted slight cross-reactivity with H-Ras and very low N-Ras recognition, but overall found it excellent for detecting endogenous K-Ras in whole cell lysates.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sehbanul (Verified Customer) (07-24-2025) | The KRAS antibody (12063-1-AP, Proteintech) shows excellent specificity and strong performance in Western blot. It reliably detects endogenous KRAS with minimal cross-reactivity, making it a valuable tool for RAS pathway studies.
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Sehbanul (Verified Customer) (07-24-2025) | The KRAS antibody (12063-1-AP, Proteintech) shows excellent specificity and strong performance in Western blot. It reliably detects endogenous KRAS with minimal cross-reactivity, making it a valuable tool for RAS pathway studies.
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Anne (Verified Customer) (07-24-2025) | no comments
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Sophy (Verified Customer) (12-22-2022) | I have tried the KRas antibody from a lot of companies and this one from proteintech definitely has been working the best for me.
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Jonas (Verified Customer) (07-29-2022) | Antibody works as expected, dilution with 1:2000 was perfect and could even be increased to 1:5000; delivery was very fast, would recommend!!
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Usman Shah (Verified Customer) (11-29-2021) | Enables specific detection of KRAS in WBs.
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Kishor (Verified Customer) (12-13-2018) | I am very much satisfied with this antibody. I got good results for cells and rat liver tissue protein.
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Christopher (Verified Customer) (12-11-2018) | Antibody is much better than comparable antibodies. Because of weak expression an very low differences between K-Ras/H-Ras/N-Ras it's quite difficult to detect only K-Ras in a specific way. That is also the problem of this antibody. Please look at my attached blot. 1. Fermentas prestained marker2. PANC-1 whole cell lysate3. PANC-1/EGFP-K-Ras4. HEK293/EGFP-K-Ras5. HEK293/EGFP-H-Ras6.HEK293/EGFP-N-Ras7. 20 ng recombinant K-Ras (His-tagged)Unfortunately, K-Ras (12063-1-AP) recognizes also H-Ras and in less amounts N-Ras. Looking at endogenous K-Ras detection, you have to keep in mind, that the upper band is K-Ras and the lower one H-Ras. Because of the fact, that N-Ras is only recognized in very low amounts (probably almost not on endogenous level) this antibody is great detecting endogenous K-Ras in whole cell lysates with some little problems
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