Validation Data Gallery
|Positive WB detected in||HeLa cells, NIH/3T3 cells, human cerebellum tissue, mouse skeletal muscle tissue|
|Positive IHC detected in||human kidney tissue, human heart tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
16094-1-AP targets KPTN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KPTN fusion protein Ag8785|
|Full Name||kaptin (actin binding protein)|
|Calculated molecular weight||436 aa, 48 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC009249|
|Gene ID (NCBI)||11133|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Kaptin, also named as KPTN and Actin-associated protein 2E4, originally isolated from human blood platelets and likely to be involved in the actin rearrangements occurring during activation. It plays a unique role in the actin rearrangements that accompany platelet activation and stereocilia formation. Kaptin is necessary for normal neuromorphogenesis. It may play a role in producing the sensory apparatus in hair cells.
Structure of the nutrient-sensing hub GATOR2.
Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes.
KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1.
CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth
The SZT2 Interactome Unravels New Functions of the KICSTOR Complex.
Dihydroxyacetone phosphate signals glucose availability to mTORC1.