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  • KD/KO Validated

KPTN Polyclonal antibody

KPTN Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16094-1-AP

Synonyms

2E4, Actin associated protein 2E4, Kaptin, kaptin (actin binding protein), KPTN



Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, human cerebellum tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman kidney tissue, human heart tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16094-1-AP targets KPTN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KPTN fusion protein Ag8785
Full Name kaptin (actin binding protein)
Calculated Molecular Weight 436 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC009249
Gene Symbol KPTN
Gene ID (NCBI) 11133
RRIDAB_2134007
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kaptin, also named as KPTN and Actin-associated protein 2E4, originally isolated from human blood platelets and likely to be involved in the actin rearrangements occurring during activation. It plays a unique role in the actin rearrangements that accompany platelet activation and stereocilia formation. Kaptin is necessary for normal neuromorphogenesis. It may play a role in producing the sensory apparatus in hair cells.

Protocols

Product Specific Protocols
WB protocol for KPTN antibody 16094-1-APDownload protocol
IHC protocol for KPTN antibody 16094-1-APDownload protocol
IF protocol for KPTN antibody 16094-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Structure of the nutrient-sensing hub GATOR2.

Authors - Max L Valenstein
humanWB

Nature

Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes.

Authors - Kuang Shen
humanWB

Nature

KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1.

Authors - Rachel L Wolfson
  • KD Validated
humanWB

Cell Rep

CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth

Authors - Shasha Yin
  • KO Validated
humanWB

Cells

The SZT2 Interactome Unravels New Functions of the KICSTOR Complex.

Authors - Cecilia Cattelani
humanWB

Nat Metab

Dihydroxyacetone phosphate signals glucose availability to mTORC1.

Authors - Jose M Orozco