KPNA4 Recombinant antibody, PBS Only

KPNA4 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230098H3

Cat no : 82898-1-PBS

Synonyms

QIP1, KPNA 4, Karyopherin subunit alpha-4, IPOA3, Importin subunit alpha-3

---

Validation Data Gallery

• 

  IF Staining of U2OS using 82898-1-RR (same clone as 82898-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KPNA4 antibody (82898-1-RR, Clone: 230098H3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82898-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 82898-1-RR (same clone as 82898-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.13 ug Anti-Human KPNA4 (82898-1-RR, Clone:230098H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.13 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82898-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82898-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82898-1-RR against Human KPNA4 were performed. The affinity constant is 0.417 nM.

---

Product Information

82898-1-PBS targets KPNA4 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KPNA4 fusion protein Ag3133
• Full Name: karyopherin alpha 4 (importin alpha 3)
• Calculated Molecular Weight: 521 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC034493
• Gene Symbol: KPNA4
• Gene ID (NCBI): 3840
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.