KPNA4 Polyclonal antibody

• Featured Product
• KD/KO Validated

KPNA4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 12463-1-AP

Synonyms

SRP3, QIP1, KPNA 4, Karyopherin subunit alpha-4, IPOA3

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Validation Data Gallery

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  WB analysis using 12463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12463-1-AP (KPNA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12463-1-AP (KPNA4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 12463-1-AP

  IP result of anti-KPNA4 (IP:12463-1-AP, 4ug; Detection:12463-1-AP 1:600) with A549 cells lysate 2000ug.

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  IHC staining of human bowen disease using 12463-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12463-1-AP (KPNA4 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12463-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12463-1-AP (KPNA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 12463-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using KPNA4 antibody (12463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: A549 cells, NIH/3T3 cells, HeLa cells, Jurkat cells, mouse testis tissue, rat testis tissue
• Positive IP detected in: A549 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• CoIP: See 1 publications below

Product Information

12463-1-AP targets KPNA4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KPNA4 fusion protein Ag3133
• Full Name: karyopherin alpha 4 (importin alpha 3)
• Calculated Molecular Weight: 521 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC034493
• Gene Symbol: KPNA4
• Gene ID (NCBI): 3840
• RRID: AB_2133821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KPNA4, also named importin subunit alpha-3, karyopherin-alpha4, is a cytoplasmic protein that recognizes nuclear localization signals (NLSs) and dock NLS-containing proteins to the nuclear pore complex. Nuclear import, mediated in part by karyopherin-α (KPNA)/importin-α subtypes, regulates transcription factor access to the genome and determines cell fate. KPNA4-mediated nuclear transport of Ras-responsive element-binding protein (RREB1), which sustains Ras/ERK pathway signaling through repressing miR-143/145 expression (PMID: 31822798). KPNA4 is one of the main isoforms that is activated in many human cancers. KPNA4 expression was elevated in head and neck of squamous cell carcinoma (PMID: 33188837).

Publications

Species	Application	Title
human	WB,CoIP	Virol Sin; African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.; Authors - Yisha Zhuo
human	WB	Oncogene; The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.; Authors - Kai Li ; KD Validated
mouse	WB	J Neurosci; The subcellular distribution of HDAC1 in neurotoxic conditions is dependent on serine phosphorylation.; Authors - Yunjiao Zhu
mouse	WB	Exp Gerontol; Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.; Authors - Shama R Iyer
human	WB	Tumour Biol; Reanalysis of microRNA expression profiles identifies novel biomarkers for hepatocellular carcinoma prognosis.; Authors - Zhengqiang Wang
human	WB	Viruses; Ivermectin Inhibits HBV Entry into the Nucleus by Suppressing KPNA2; Authors - Anna Nakanishi