KPNA3 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

KPNA3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D3D2

Cat no : 67892-1-PBS

Synonyms

hSRP1, Importin alpha Q2, Importin subunit alpha 3, IPOA4, Karyopherin subunit alpha 3, KPNA3, Qip2, SRP1, SRP1 gamma, SRP1gamma, SRP4

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Validation Data Gallery

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  WB analysis using 67892-1-Ig (same clone as 67892-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67892-1-Ig (KPNA3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67892-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67892-1-Ig (same clone as 67892-1-PBS)

  WB result of KPNA3 antibody (67892-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KPNA3 transfected HepG2 cells. This data was developed using the same antibody clone with 67892-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67892-1-Ig (same clone as 67892-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67892-1-Ig (KPNA3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67892-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 67892-1-Ig (same clone as 67892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67892-1-Ig (KPNA3 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67892-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 67892-1-Ig (same clone as 67892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67892-1-Ig (KPNA3 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67892-1-PBS in a different storage buffer formulation.

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Product Information

67892-1-PBS targets KPNA3 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: KPNA3 fusion protein Ag27767
• Full Name: karyopherin alpha 3 (importin alpha 4)
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC017355
• Gene Symbol: KPNA3
• Gene ID (NCBI): 3839
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

karyopherin subunit alpha 3 (KPNA3) is a member of the importin alpha family which can transport molecules between the mucleus and cytoplasm. It is ubiquitous expressed in brain,testis and esophagus. KPNA3 is associated with many severe diseases such as hepatocellular Carcinoma (PMID: 31417635) , schizophrenia, major depression and opiate dependence (PMID: 22960338) . The molecular weight of KPNA3 is about 58 kDa.