KPNA2 Polyclonal antibody

• Featured Product
• KD/KO Validated

KPNA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

42

Reviews (2)

Cat no : 10819-1-AP

Synonyms

KPNA 2, Karyopherin subunit alpha-2, IPOA1, IPOA 1, IPO A1

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Validation Data Gallery

• 

  WB analysis using 10819-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10819-1-AP (KPNA2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10819-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10819-1-AP (KPNA2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10819-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10819-1-AP (KPNA2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10819-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KPNA2 antibody (10819-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, Raji cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 34 publications below
• IHC: See 13 publications below
• IF: See 8 publications below
• IP: See 3 publications below
• CoIP: See 6 publications below
• ChIP: See 1 publications below

Product Information

10819-1-AP targets KPNA2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KPNA2 fusion protein Ag1153
• Full Name: karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
• Calculated Molecular Weight: 57.8 kDa
• Observed Molecular Weight: 55-58 kDa
• GenBank Accession Number: BC005978
• Gene Symbol: KPNA2
• Gene ID (NCBI): 3838
• RRID: AB_2265526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KPNA2 (karyopherin α2) is a nuclear import factor involved in the nucleocytoplasmic transport system. It is functionally involved in the tumor progression and is elevated in multiple cancers. KPNA2 expression may be a useful prognostic biomarker to monitor cancer prognosis.

Publications

Species	Application	Title
human	WB	Cell Res; Neddylation of PTEN regulates its nuclear import and promotes tumor development.; Authors - Ping Xie
human	WB,CoIP	J Exp Clin Cancer Res; ISG15 and ISGylation modulates cancer stem cell-like characteristics in promoting tumor growth of anaplastic thyroid carcinoma; Authors - Tong Xu ; KD Validated
human	WB, IP	Cell Death Differ; lncRNA THAP7-AS1, transcriptionally activated by SP1 and post-transcriptionally stabilized by METTL3-mediated m6A modification, exerts oncogenic properties by improving CUL4B entry into the nucleus.; Authors - Hai-Ting Liu
human	WB	Mol Cell Proteomics; Quantitative proteomics reveals regulation of karyopherin subunit alpha-2 (KPNA2) and its potential novel cargo proteins in nonsmall cell lung cancer.; Authors - Wang Chun-I CI ; KD Validated
human	WB,CoIP	Virol Sin; African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.; Authors - Yisha Zhuo
human	WB,IHC	Oncogene; USP1 inhibition destabilizes KPNA2 and suppresses breast cancer metastasis.; Authors - Aihui Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

María (Verified Customer) (02-08-2022)

Works for WB (1:1000) and IF in permeabilized cells (1:100). Validated with siRNA

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RPE-1/MSC

FH

Chandra (Verified Customer) (09-16-2019)

Works best at a 1:1000 antibody dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human cancer cell lines

KPNA2 Antibody Western Blot, validation (1:1000 dilution) in human cancer cell lines (Cat no:10819-1-AP)

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