KNTC1 Polyclonal antibody

KNTC1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24694-1-AP

Synonyms

Kinetochore-associated protein 1, Kinetochore associated protein 1

---

Validation Data Gallery

• 

  WB analysis using 24694-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24694-1-AP (KNTC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, NCI-H1299 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24694-1-AP targets KNTC1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KNTC1 fusion protein Ag20268
• Full Name: kinetochore associated 1
• Calculated Molecular Weight: 251 kDa
• Observed Molecular Weight: 210-230 kDa
• GenBank Accession Number: BC150278
• Gene Symbol: KNTC1
• Gene ID (NCBI): 9735
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KNTC1 is a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. Northern blot analysis revealed low-to-moderate expression in all tissues tested, with highest expression in testis.