KMT2C Recombinant antibody

KMT2C Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240542A2

Cat no : 83595-4-RR

Synonyms

MLL3, Lysine N methyltransferase 2C, KIAA1506, Homologous to ALR protein, HALR

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Validation Data Gallery

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  IF Staining of MCF-7 using 83595-4-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KMT2C antibody (83595-4-RR, Clone: 240542A2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (green).

• 

  FC experiment of U2OS using 83595-4-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug KMT2C Recombinant antibody (83595-4-RR, Clone:240542A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83595-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83595-4-RR against Human KMT2C were performed. The affinity constant is 2.67 nM.

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83595-4-RR is KMT2C Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KMT2C fusion protein Ag28875
• Full Name: myeloid/lymphoid or mixed-lineage leukemia 3
• Calculated Molecular Weight: 541 kDa
• GenBank Accession Number: NM_170606
• Gene Symbol: KMT2C
• Gene ID (NCBI): 58508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lysine (K)-specific methyltransferase 2C (KMT2C, also known as MLL3) belongs to the mixed-lineage leukemia (MLL) family of histone methyltransferases which methylate the histone 3 tail at lysine 4 (H3K4) as part of the complex proteins associated with Set 1 (COMPASS) complex. Although originally identified as oncogenic fusions in leukemia, genome-wide mutation studies have revealed frequent, presumably loss-of-function, mutations in various members of the MLL family, including MLL2/KMT2B, MLL3/KMT2C, and MLL4/KMT2D in a variety of malignancies, particularly solid tumors. Mechanistic studies of KMT2C in normal cells have focused primarily on its role in enhancer regulation by deposition of H3K4me1 marks.