KMO Monoclonal antibody

KMO Monoclonal Antibody for WB, ELISA
Cat No. 60029-1-Ig
Clone No.2D2F2

Host / Isotype

Mouse / IgG1

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

KMO, Kynurenine 3 hydroxylase, Kynurenine 3 monooxygenase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inPC-3 cells, SKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60029-1-Ig targets KMO in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KMO fusion protein Ag1109 Predict reactive species
Full Name kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
Calculated Molecular Weight486 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC005297
Gene Symbol KMO
Gene ID (NCBI) 8564
RRIDAB_2133396
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15229
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KMO(Kynurenine 3-monooxygenase) is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).

Protocols

Product Specific Protocols
WB protocol for KMO antibody 60029-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, mouseIF

J Am Soc Nephrol

Loss of Kynurenine 3-Mono-oxygenase Causes Proteinuria.

Authors - Ron Korstanje
humanIHC

Neuro Oncol

Molecular imaging correlates of tryptophan metabolism via the kynurenine pathway in human meningiomas.

Authors - Edit Bosnyák
humanIHC

Oncol Lett

Viability of diffuse large B-cell lymphoma cells is regulated by kynurenine 3-monooxygenase activity

Authors - NANAKA MORITA
mouseWB

Hum Mol Genet

Retention of stress susceptibility in the mdx mouse model of Duchenne muscular dystrophy after PGC-1α overexpression or ablation of IDO1 or CD38

Authors - Erynn E Johnson
mouseIHC

Br J Pharmacol

Chronic stress induces behavioural changes through increased kynurenic acid by downregulation of kynurenine-3-monooxygenase with microglial decline

Authors - Masaya Hasegawa
ratWB

J Pharmacol Sci

Thrombin-induced kynurenine 3-monooxygenase causes variations in the kynurenine pathway, leading to neurological deficits in a murine intracerebral hemorrhage model

Authors - Masatoshi Ohnishi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Hala (Verified Customer) (06-07-2022)

Works very good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: Huh6 and Hepg2
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