KLRB1/CD161 Monoclonal antibody

KLRB1/CD161 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3D8F5

Cat no : 67537-1-Ig

Synonyms

CD161, CLEC5B, hNKR P1A, KLRB1, NKR, NKR P1, NKR P1A, NKRP1A



Tested Applications

Positive WB detected inhuman spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67537-1-Ig targets KLRB1/CD161 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KLRB1/CD161 fusion protein Ag27117
Full Name killer cell lectin-like receptor subfamily B, member 1
Calculated Molecular Weight 225 aa, 25 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC114516
Gene Symbol CD161
Gene ID (NCBI) 3820
RRIDAB_2882756
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD161, also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).

Protocols

Product Specific Protocols
WB protocol for KLRB1/CD161 antibody 67537-1-IgDownload protocol
IHC protocol for KLRB1/CD161 antibody 67537-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Rep Med

Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers

Authors - Bingxin Liu
humanWB

J Thorac Dis

KLRB1 expression is associated with lung adenocarcinoma prognosis and immune infiltration and regulates lung adenocarcinoma cell proliferation and metastasis through the MAPK/ERK pathway

Authors - Siwei Xu
humanWB

Front Endocrinol (Lausanne)

Machine learning immune-related gene based on KLRB1 model for predicting the prognosis and immune cell infiltration of breast cancer

Authors - Guo Huang
humanIHC

BMJ Open Respir Res

Single-cell RNA-sequencing uncovers the dynamic changes of tumour immune microenvironment in advanced lung adenocarcinoma

Authors - Haijiao Lu
humanIHC

Front Immunol

Single-cell sequencing analysis and multiple machine-learning models revealed the cellular crosstalk of dendritic cells and identified FABP5 and KLRB1 as novel biomarkers for psoriasis

Authors - Zhiqiang Ma
humanIF,IHC

J Pathol Clin Res

Immune checkpoint CD161/LLT1-associated immunological landscape and diagnostic value in oral squamous cell carcinoma

Authors - Xinyang Hu