KLRB1/CD161 Monoclonal antibody

KLRB1/CD161 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

3D8F5

Cat no : 67537-1-Ig

Synonyms

CD161, CLEC5B, hNKR P1A, KLRB1, NKR, NKR P1, NKR P1A, NKRP1A

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Validation Data Gallery

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  WB analysis of human spleen using 67537-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 67537-1-Ig (KLRB1/CD161 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 67537-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67537-1-Ig (KLRB1/CD161 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67537-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67537-1-Ig (KLRB1/CD161 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67537-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67537-1-Ig (KLRB1/CD161 antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human spleen tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

67537-1-Ig targets KLRB1/CD161 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KLRB1/CD161 fusion protein Ag27117
• Full Name: killer cell lectin-like receptor subfamily B, member 1
• Calculated Molecular Weight: 225 aa, 25 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC114516
• Gene Symbol: CD161
• Gene ID (NCBI): 3820
• RRID: AB_2882756
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD161, also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).

Publications

Species	Application	Title
human	IF	Cell Rep Med; Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers; Authors - Bingxin Liu
human	WB	J Thorac Dis; KLRB1 expression is associated with lung adenocarcinoma prognosis and immune infiltration and regulates lung adenocarcinoma cell proliferation and metastasis through the MAPK/ERK pathway; Authors - Siwei Xu
human	WB	Front Endocrinol (Lausanne); Machine learning immune-related gene based on KLRB1 model for predicting the prognosis and immune cell infiltration of breast cancer; Authors - Guo Huang
human	IHC	BMJ Open Respir Res; Single-cell RNA-sequencing uncovers the dynamic changes of tumour immune microenvironment in advanced lung adenocarcinoma; Authors - Haijiao Lu
human	IHC	Front Immunol; Single-cell sequencing analysis and multiple machine-learning models revealed the cellular crosstalk of dendritic cells and identified FABP5 and KLRB1 as novel biomarkers for psoriasis; Authors - Zhiqiang Ma
human	IF,IHC	J Pathol Clin Res; Immune checkpoint CD161/LLT1-associated immunological landscape and diagnostic value in oral squamous cell carcinoma; Authors - Xinyang Hu