KLK4 Polyclonal antibody

KLK4 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26547-1-AP

Synonyms

ARM1, EMSP, EMSP1, Kallikrein 4, Kallikrein like protein 1, kallikrein related peptidase 4, KLK L1, KLK4, PROSTASE, PRSS17, PSTS, Serine protease 17

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Validation Data Gallery

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  IP experiment of DU 145 using 26547-1-AP

  IP result of anti-KLK4 (IP:26547-1-AP, 4ug; Detection:26547-1-AP 1:300) with DU 145 cells lysate 2200ug.

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  IHC staining of human prostate hyperplasia using 26547-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 26547-1-AP (KLK4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 26547-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 26547-1-AP (KLK4 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IP detected in: DU 145 cells
• Positive IHC detected in: human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 26547-1-AP is KLK4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLK4 fusion protein Ag24240
• Full Name: kallikrein-related peptidase 4
• Calculated Molecular Weight: 254 aa, 27 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC069325
• Gene Symbol: KLK4
• Gene ID (NCBI): 9622
• RRID: AB_2880548
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Am J Pathol; Kallikrein-Related Peptidase 4 Promotes Proliferation, Migration, Invasion, and Pro-Angiogenesis of Endometrial Stromal Cells via Regulation of Brain-Derived Neurotrophic Factor Production in Endometriosis; Authors - Jingwen Shi