KLK3/PSA Monoclonal antibody, PBS Only

KLK3/PSA Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A1E2

Cat no : 60338-1-PBS

Synonyms

APS, Gamma seminoprotein, hK3, Kallikrein 3, kallikrein related peptidase 3, KLK2A1, KLK3, KLK3,PSA, KLK3/PSA, P 30 antigen, Prostate specific antigen, PSA, Semenogelase, Seminin

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Validation Data Gallery

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  WB analysis using 60338-1-Ig (same clone as 60338-1-PBS)

  PC-3(PSA-), DU 145(PSA-) and LNCaP (PSA+) cell lysates were subjected to SDS PAGE followed by western blot with 60338-1-Ig (KLK3/PSA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60338-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60338-1-Ig (same clone as 60338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60338-1-Ig (KLK3/PSA antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60338-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60338-1-Ig (same clone as 60338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60338-1-Ig (KLK3/PSA antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60338-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60338-1-Ig (same clone as 60338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60338-1-Ig (KLK3,PSA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60338-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60338-1-Ig (same clone as 60338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60338-1-Ig (KLK3,PSA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60338-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60338-1-Ig (same clone as 60338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using (KLK3,PSA Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60338-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60338-1-Ig (same clone as 60338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using (KLK3,PSA Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60338-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60338-1-PBS targets KLK3/PSA in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KLK3/PSA fusion protein Ag1060
• Full Name: kallikrein-related peptidase 3
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC005307
• Gene Symbol: KLK3
• Gene ID (NCBI): 354
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms produced by alternative splicing.